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  • 2000-2004  (4)
  • 1
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Sensitization to natural rubber latex (Hevea brasiliensis) is a major cause of occupational asthma and rhinitis affecting frequent latex-glove users. Hev b 6.01, a known major latex allergen, is cleaved naturally into hevein (4.7 kDa) and a C-terminal fragment (14 kDa). Hevein is an abundant protein in latex-glove extracts. As the immune response to allergens is initiated by activation of allergen-specific CD4+ T cells, identification of dominant T cell epitopes is crucial for the development of specific immunotherapy.Objective To identify dominant T cell epitopes of Hev b 6.01 in latex-allergic glove users.Methods Ten latex-allergic frequent glove users and six non-latex-allergic atopic control subjects were selected, based on clinical symptoms and positive latex-specific serum IgE. Serum IgE reactivity to glove extract and recombinant Hev b 6.01 (rHev b 6.01) were analysed by ELISA. Latex-specific short-term oligoclonal T cell lines were generated from peripheral blood of latex-allergic subjects. These lines were tested for proliferative responses to overlapping 20-mer peptides of the Hev b 6.01 molecule. CD4+ T cell intracellular cytokines, IL-4 and IFN-γ were assessed following stimulation with immobilized anti-CD3 in the presence of IL-2.Results All ten of the latex-allergic patients showed serum IgE binding to glove extract while eight of these also showed IgE binding to rHev b 6.01 by ELISA. Western blotting confirmed reactivity with rHev b 6.01 at around 20 kDa. T cell proliferation assays showed that latex-specific T cell lines from all subjects responded to one or more peptides, with greatest frequency of reactivity to peptides Hev b 6.01 p(10–29) and Hev b 6.01 p(19–38) in the hevein domain. An allergic-type cytokine profile with considerable IL-4 in addition to IFN-γ was evident from intracellular cytokine staining.Conclusion Hevein is an important T cell as well as B cell immunogen and contains dominant T cell reactive sites.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0630
    Keywords: PACS: 85.42.+m; 85.40.Vb; 85.40.Tv
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. Memories that utilize single-electron effects are an attempt at combining the discreteness observable in transport of electrons on to very small capacitances (∼10-18 F) and into three-dimensionally quantum-confined states, with the reproducibility, architecture and integration of the field-effect devices. We discuss the role size plays in the operation and its variability for such memories. In particular, we discuss the implications of size effects through barriers on speed; through electrostatics on variability, acceptability and reproducibility of properties desired; through random variations and of tunneling on limits in the use of the field-effect, and through interface-states on the time-domain operation. For device properties and their variations, using silicon-on-insulator substrates, silicon and back-insulator thicknesses matter through the linear variations introduced in the electrostatic potential and quadratic variations introduced in the subband energies, the quantum-dots and nano-crystals matter secondarily through the electrostatics and the linear dependence of capacitance on size and the quadratic dependence of the allowed eigen-energies on size. We also discuss the implications of tunneling on time constants of charging of the confined states and in between the source and the drain for the ultimate structure size limit.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 455-456 (May 2004), p. 472-475 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 455-456 (May 2004), p. 587-591 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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