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  • 2000-2004  (5)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Matrix metalloproteinases and their inhibitors in oral lichen planus (2001)
    Copenhagen : Munksgaard International Publishers
    Journal of cutaneous pathology 28 (2001), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Oral lichen planus (OLP) is characterized by a sub-epithelial lymphocytic infiltrate, basement membrane (BM) disruption, intra-epithelial T-cell migration and apoptosis of basal keratinocytes. BM damage and T-cell migration in OLP may be mediated by matrix metalloproteinases (MMPs).Methods: We examined the distribution, activation and cellular sources of MMPs and their inhibitors (TIMPs) in OLP using immunohistochemistry, ELISA, RT-PCR and zymography.Results: MMP-2 and -3 were present in the epithelium while MMP-9 was associated with the inflammatory infiltrate. MMP-9 and TIMP-1 secretion by OLP lesional T cells was greater than OLP patient (p〈0.01) and healthy control subject (p〈0.001) peripheral blood T cells. MMP-9 and TIMP-1 mRNA levels were greater in OLP lesional T cells compared with healthy control subject peripheral blood T cells (p〈0.01). Tumor necrosis factor (TNF)-α upregulated OLP lesional T-cell MMP-9 (not TIMP-1) mRNA and secretion (p〈0.05). The in vitro activation rate of MMP-9 from OLP lesional T cells was greater than that from OLP peripheral blood T cells (p〈0.05).Conclusion: T-cell-derived MMP-9 may be involved in the pathogenesis of OLP. Relative over-expression of MMP-9 (compared with TIMP-1) may cause BM disruption and facilitate intra-epithelial T-cell migration in OLP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0560.2001.280203.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Th1 cytokines in oral lichen planus (2003)
    Oxford, UK : Munksgaard International Publishers
    Journal of oral pathology & medicine 32 (2003), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Cell-mediated immune responses in oral lichen planus (OLP) may be regulated by cytokines and their receptors.Methods:  In situ cytokine expression and in vitro cytokine secretion in OLP were determined by immunohistochemistry and ELISA.Results:  The majority of subepithelial and intraepithelial mononuclear cells in OLP were CD8+. In some cases, intraepithelial CD8+ cells were adjacent to degenerating keratinocytes. CD4+ cells were observed mainly in the deep lamina propria with occasional CD4+ cells close to basal keratinocytes. Mononuclear cells expressed IFN-γ in the superficial lamina propria and TNF-α adjacent to basal keratinocytes. Basal keratinocytes expressed TNF-α as a continuous band. TNF R1 was expressed by mononuclear cells and basal and suprabasal keratinocytes. There was variable expression of TGF-β1 in the subepithelial infiltrate while all intraepithelial mononuclear cells were TGF-β1−. Keratinocytes in OLP stained weakly for TGF-β1. Unstimulated OLP lesional T cells secreted IFN-γin vitro. TNF-α stimulation down-regulated IFN-γ secretion and up-regulated TNF-α secretion. IL-4, IL-10 and TGF-β1 secretion were not detected.Conclusions:  These data suggest the development of a T helper 1 immune response that may promote CD8+ cytotoxic T-cell activity in OLP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0714.2003.00077.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A fluorometric microassay for histamine release from human gingival mast cells (2001)
    Copenhagen : Munksgaard International Publishers
    Journal of periodontal research 36 (2001), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Mast cells are important effector cells of the immune system. We describe a rapid and inexpensive microassay to determine histamine release from human gingival mast cells. The assay is based on the coupling of histamine with o-phthalaldehyde (OPT) at a highly alkaline pH to form a fluorescent product. Using this assay with a sample volume of 10 μl/well in a 384 black well microplate, the histamine detection limit was 0.031 μg/ml. The human mast cell line (HMC-1) and fresh mast cells isolated from human gingival tissue (n=10) were stimulated with substance P, anti-IgE or calcium ionophore A23187. Calcium ionophore significantly increased histamine release from HMC-1 cells and gingival mast cells (p〈0.05). This microassay will facilitate the study of mast cell histamine release in diseased oral mucosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0765.2001.036004233.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Intra-epithelial CD8+ T cells and basement membrane disruption in oral lichen planus (2002)
    Oxford, UK : Blackwell Science, Ltd
    Journal of oral pathology & medicine 31 (2002), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  We investigated basement membrane (BM) disruption and the distribution of mast cells (MCs) and T cell subsets, in oral lichen planus (OLP) and normal buccal mucosa (NBM) using immunohistochemistry. In OLP, there were increased numbers of tryptase+ MCs in areas of BM disruption (P 〈 0.05).Method:  We identified clusters of intraepithelial CD8+ T cells in OLP, specifically in regions of BM disruption. The number of intraepithelial CD8+ T cells in regions of BM disruption was significantly greater than in regions of BM continuity (P 〈 0.05).Results:  There were comparable numbers of lamina propria CD8+ T cells in regions of BM disruption and BM continuity. The number of CD4+ T cells in the epithelium and lamina propria of OLP lesions did not vary between regions of BM disruption and BM continuity.Conclusion:  These data suggest a role for MCs in epithelial BM disruption in OLP. CD8+ T cells may migrate through BM breaks to enter the OLP epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0904-2512.2001.10063.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular and cell biology of the gingiva (2000)
    Copenhagen : Munksgaard International Publishers
    Periodontology 2000 24 (2000), S. 0 
    Details
    ISSN: 1600-0757
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0757.2000.2240103.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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