ISSN:
1573-2568
Keywords:
COMPOUND 40/80
;
MAST CELL DEGRANULATOR
;
GASTRIC MUCOSA
;
MUCOSAL LESION RAT
;
ACTIVE OXYGEN METABOLISM
;
OXIDATIVE STRESS
;
BLOOD FLOW
;
ISCHEMIA-REPERFUSION
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The relationship between the changes of activeoxygen metabolism and blood flow and the formation,progression, and recovery of lesions was examined in thegastric mucosa of rats treated once with compound 48/80, a mast cell degranulator. Gastricmucosal lesions appeared 0.5 hr after compound 48/80treatment, became worst at 3 hr, and recovered fairlywell at 12 hr. Increases in gastric mucosal lipidperoxide content and xanthine oxidase andmyeloperoxidase activities and decreases in gastricmucosal vitamin E and hexosamine contents andSe-dependent glutathione peroxidase activity occurredwith the formation and progression of gastric mucosal lesions.These changes were attenuated with the recovery of thelesion. Gastric mucosal nonprotein SH content decreasedwith the formation of gastric mucosal lesions, and this decreased SH content returned to nearthe original level with lesion progression. No changesin gastric mucosal superoxide dismutase and catalaseactivities occurred with the formation, progression, and recovery of gastric mucosal lesions.Gastric mucosal blood flow decreased with the formationof gastric mucosal lesions, and this decreased bloodflow recovered with lesion progression. Serum serotonin concentration, an index of mast celldegranulation, increased with the formation of gastricmucosal lesions, and this increased serotonin level wasattenuated with lesion progression and recovery.Pretreatment with ketotifen, a connective tissue mast cellstabilizer, prevented the formation of gastric mucosallesions, the increases of gastric mucosal lipid peroxidecontent, xanthine oxidase and myeloperoxidase activities, and serum serotonin level; and thedecreases of gastric mucosal nonprotein SH content,glutathione peroxidase activity, and blood flow found at0.5 hr after compound 48/80 treatment. These results indicate that the changes of gastric mucosalactive oxygen metabolism and blood flow are closelyrelated to the formation, progression, and recovery ofgastric mucosal lesions in rats with a single compound 48/80 treatment. The present results alsosuggest that this compound 48/80-induced gastric mucosalinjury could be a kind of ischemia-reperfusion-inducedinjury occurring through degranulation of connective tissue mast cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018854107623
Permalink
