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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 49 (1984), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Power and cooking time combinations necessary to devitalize trichinae in pork roasts were determined using three power levels in each of two microwave ovens. The combinations obtained for an oven listed as producing 700 watts were: 100% power, 19.8 min/kg; 70%, 24.2 min/kg; and 30%. 55.0 min/kg. Those for a listed 625 watt oven were: 100% power, 24.2 min/kg; 70%, 26.4 min/kg; and 30%, 55.0 min/kg. Inadequate doneness in many roasts cooked with the safe combinations would necessitate additional cooking time before procedures could be recommended. Post-cooking temperatures during a 20-min standing period may either increase or decrease with no predictability.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 49 (1984), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sixteen trichina-infected pork roasts were cooked at 30% power in two microwave ovens for 48.4 min/kg. Seven roasts attained or closely approached 76.7°C thoughout and were not recooked. Nine roasts whose initial post-cooking low temperatures varied from 42.8–72.8°C were recooked one to three times at 8.8 min/kg. One of the roasts recooked three times probably would have required two more recookings at 8.8 min/kg to obtain 76.7°C throughout. Eight of the 16 roasts were underdone and should have been recooked. Trichinae in all sixteen roasts, irrespective of temperatures or degree of doneness attained, were noninfective to rats.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture nutrition 3 (1997), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The availability of a rapid, cost-effective, accurate and reliable method of assessing fish meal protein digestibility would greatly enhance and standardize quality-control procedures in both the fish meal and fish feed industries. However, while several in vitro digestibility tests have been developed, few have been adopted by industry due to their time-consuming nature, problems surrounding reliability and/or inconsistencies in predictive ability. The present investigation was undertaken to evaluate the utility and predictive qualities of two in vitro digestion assays of distinct design. Methods examined included a novel open system, wherein on-line removal of digestion products was attainable, and a closed system, which permitted analysis of products following completion of the digestion process. Results provided by the two systems were compared using four differentially processed fish meals. The open system supplied information based upon the detected quantity of products below 10 kDa. The closed system provided measurement of free amino groups. Both methods were in agreement with respect to assessing the presence of hydrolysed product. Both systems furnished complementary data with respect to the characterization of fish meal protein quality. The closed and open systems provided insight upon digestibility and digestion kinetic profiles respectively.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 1224-1225 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Three β-mannanase isoforms were isolated from the supernatant of a thermophilic actinomycete culture from Thermomonospora fusca KW3. Two of the isoforms (Q1, Q 1.1) were crystallized by the hanging-drop method at room temperature using ammonium sulfate as a precipitant. The isoforms form rod-shaped colorless crystals. Both belong to the orthorhombic space group P212121. The cell dimensions are a = 46.7, b = 61.1, and c = 128.2 Å for isoform Q1, and a = 43.8, b = 46.2, and c = 132.8 Å for isoform Q1.1. The asymmetric unit of either isoform contains one mannanase molecule. Native data have been collected to 2.2 Å resolution for Q1 and to 1.65 Å resolution for Q1.1 using synchrotron radiation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 48 (1983), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pork roasts from pigs experimentally infected with Trichinella spiralis were cooked in five microwave ovens. Twenty-nine procedures, generally recommended by the oven manufacturers or pork interest groups, were used. Infective trichinae were present after cooking in 50 of 189 roasts. Factors affecting infectivity included procedure used, use or nonuse of post-cooking standing or temperature holding, weight of roast, and internal temperatures attained. Five visually well done roasts contained infective trichinae including one in which all five temperatures taken exceeded 76.7°C. One other infective roast, with a small red spot, also had all temperatures at or above the 76.7°C level. Recommendations are made to minimize the potential that trichinae may survive in microwaved-cooked pork.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 48 (1983), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pork roasts, obtained from pigs experimentally infected with Trichinella spiralis, were cooked at 50% power in four microwave ovens. The attainment of consistently well done roasts is the primary requisite for development of safe cooking methods. Recommendations for cooking of trichinous pork roasts, with adequate margins of safety, are made for each oven.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 236 (1982), S. 237-244 
    ISSN: 1434-4726
    Keywords: Lärm ; Anaesthetika ; Meerschweinchen ; Mikrophonpotentiale ; Nervenaktionspotentiale ; Hirnstammpotentiale ; hyperbarer Sauerstoff ; Noise ; Anesthetics ; Guinea pigs ; Cochlear microphonics ; Action potential of auditory nerve ; Brain stem response ; Oxygen under high pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The effect of hyperbaric oxygen on cochlear microphonics, action potential of the auditory nerve, and brain stem response damaged by short exposure to noise is varied. Of 26 guinea pigs 14 showed a positive influence as measured by amplitudes. In 12 animals oxygen had little or no effect. The reason for this could lie in the individual reaction to the anesthetic of the section of the auditory nerve tested. The reaction of postmortem cochlear microphonics after hyperbaric oxygen treatment indicates oxygen diffusion through the round window.
    Notes: Zusammenfassung Hyperbarer Sauerstoff wirkt auf die durch kurzzeitige Lärmbelästigung geschädigten Mikrophon-, Nervenaktions- und Stammhirnpotentiale unterschiedlich. Vierzehn von 26 Meerschweinchen zeigten einen positiven Effekt, — gemessen an den Amplituden. Bei 12 Tieren wirkte Sauerstoff nicht oder nur sehr gering. Die Ursache wird in individuellen Narkoseeinflüssen auf den gemessenen Hörabschnitt vermutet. Das Verhalten der postmortalen Mikrophonpotentiale nach hyperbarer Oxygenation läßt auf eine Sauerstoffdiffusion durch das runde Fenster schließen.
    Type of Medium: Electronic Resource
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  • 8
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    Unknown
    Berlin : Periodicals Archive Online (PAO)
    Deutsche Zeitschrift für Philosophie. 28:3 (1980) 373 
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  • 9
    facet.materialart.
    Unknown
    Berlin : Periodicals Archive Online (PAO)
    Deutsche Zeitschrift für Philosophie. 30:1 (1982) 120 
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  • 10
    ISSN: 1432-0983
    Keywords: Heterologous gene expression ; Plasmid copy number ; GAP promoter ; Hirudin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The yeast Saccharomyces cerevisiae has been engineered to synthesize and secrete desulfato-hirudin (hirudin), a thrombin inhibitor from the leech Hirudo medicinalis. The synthetic gene coding for hirudin was expressed constitutively under the control of four size-variants of the yeast glyceraldehyde-3-phosphate dehydrogenase promoter (GAP) and cloned into a 2 μ based multicopy yeast vector. The constitutive action of the four promoter variants was confirmed by demonstrating that the expression and secretion of hirudin is growth-related. The different efficiencies of the promoter variants not only affected hirudin expression but also led to changes in several cellular parameters, such as cell growth, average plasmid copy number and plasmid stability. The observed changes show that yeast cells establish a specific equilibrium for each promoter variant. We conclude, that the adjustment of cellular parameters in response to the expression levels of a heterologous protein is regulated by two counteracting selective forces: (1) the need for complementation of the auxotrophic host marker by the plasmid-encoded selection gene which, in the case of dLEU2, requires several plasmid copies; and (2) a selective advantage of cells with a lower copy number enabling them to escape the burden of heterologous protein production.
    Type of Medium: Electronic Resource
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