ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Flurazolidone and other nitro-pharmaceuticals
;
Quantitative analysis
;
Stability tests
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A simple, rapid and precise stability-indicating high performance liquid chromatographic assay for the determination of furazolidone (I), nitrofurazone (II), nitrofurantoin (III), niridazole (IV) and nifuroxime (V) in pure form and in pharmaceutical preparations has been devloped. Reversed phase chromatography was conducted using a Lichrosorb R.P. 18 column (250×4 mm), with methanol-water-buffer pH3 (40∶55∶5) eluent and detection at 365, 375, 367, 368 and 340 nm respectively. Calibration graphs were linear over the concentration ranges 3–18, 1–10, 1–10, 3–18 and 3–18 μm·ml−1. Reoveries from bulk drugs were 99.94–100.00 with a relative standard deviation of 1.02–1.61. Sunlight degradation of most of the studied drugs to 5-nitrofuraldehyde was observed. Accelerated stability studies have been carried out on each drug by exposure to sunlight for different time periods. Graphs of log of the remaining concentration (Log. C) against time indicated that photodecomposition of the nitrocompounds is a first order reaction. The proposed method was applied to some representative dosage forms and the results obtained were in good agreement with those obtained by the official methods.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02274542
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