ISSN:
1432-203X
Keywords:
Interspecific
;
Somatic hybrid
;
Protoplast fusion
;
Hyoscyamus muticus
;
Hyoscyamus albus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Somatic hybrid plants have been regenerated from fused protoplasts of a chlorophyll deficient mutant of H. muticus (2n=28) with wild type protoplasts of H. albus (2n=68). The inability of protoplasts of H. albus to regenerate was utilized in complementation with achlorophyllous, but regenerating, protoplasts of H. muticus for the selection of green somatic hybrid colonies and plants. The somatic hybrid plants showed intermediate morphological characters, and possessed 82–120 chromosomes, with a modal number of 96 which is also the amphidiploid complement of the two species. The isozyme patterns indicated the presence and expression of genes from both parents. The hybrid plants produced 33–78% viable pollen and set viable seeds upon selfing and backcrossing in a directional manner.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00232952
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