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  • 1995-1999  (1)
  • 1990-1994  (1)
  • Disconnected Operation  (1)
  • Rat (Wistar, Sprague-Dawley, Long Evans, Zucker)  (1)
  • 1
    ISSN: 1432-0878
    Keywords: Brain, vertebrate ; Phenylethanolamine-N-methyltransferase ; Adrenaline ; Immunocytochemistry ; HPLC ; Rat (Wistar, Sprague-Dawley, Long Evans, Zucker)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemistry was used to compare the immunoreactivity of adrenergic neurons to a well characterized specific immunoserum to phenylethanolamine-N-methyltransferase (PNMT) in different strains of rats commonly used in research studies. In adult animals, marked differences were found in the PNMT-immunoreactivity of neurons between Wistar rats and other strains, resulting in a lower PNMT-immunostaining intensity (i) within neuronal perikarya of the medulla oblongata, and (ii) more strikingly, within nerve fibers and terminals located in various brain regions. This low PNMT-immunoreactivity of nerve fibers was detected both in 14- and 35-day-old Wistar rats. On the other hand, the HPLC measurement of catecholamines, in particular of adrenaline in the hypothalamus and the medulla oblongata, did not show any difference between adult Wistar and Sprague-Dawley rats. These data suggest that the low PNMT-immunoreactivity observed in central adrenergic neurons of the Wistar rats is related to the poor recognition of the antigen by the PNMT-antibody used. Possibly, these nerve cells mainly display an isoform of the enzyme that is immunologically different from the PNMT contained within the adrenergic neurons of other rat strains.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Distributed and parallel databases 4 (1996), S. 229-247 
    ISSN: 1573-7578
    Keywords: Workflow ; Disconnected Operation ; Mobile Computing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Workflow Management Systems (WFMSs) automate the execution of business processes in environments encompassing large numbers of users distributed over a wide geographic area and using heterogeneous resources. Current implementations allow the definition and controlled execution of complex and long lived business processes as the basis for an enterprise-wide collaborative system but, in most cases, the autonomy of the users is greatly restricted due to architectural and design considerations. In particular, existing systems are built around a centralized server. As a result, users need to maintain an uninterrupted connection with the server to perform the different tasks assigned to them. This is a severe restriction, especially when considering the emergence of mobile computing, and the increase in use of laptops and small computers which are connected to the network only occasionally and which will, undoubtedly, be the tool of choice for many users. This paper addresses the problem of supporting disconnected workflow clients in large workflow management systems while still preserving the correctness of the overall execution and allowing coordinated interactions between the different users regardless of their location.
    Type of Medium: Electronic Resource
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