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  • 1995-1999  (1)
  • 1990-1994  (3)
  • dispersionpolymerization  (2)
  • α-Smooth muscle actin  (2)
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  • 1995-1999  (1)
  • 1990-1994  (3)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical identification of Ito cells and their myofibroblastic transformation in adult human liver (1994)
    Springer
    Virchows Archiv 424 (1994), S. 249-256 
    Details
    ISSN: 1432-2307
    Keywords: Adult human liver ; Ito cell ; Myofibroblast ; Immunocytochemistry ; α-Smooth muscle actin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To identify Ito cells in normal and pathological adult human livers, immunohistochemical studies were performed by the avidin-biotin-peroxidase complex method using monoclonal antibodies for α-smooth muscle actin (ASMA), desmin, and vimentin. Fifty one needle biopsies, 7 surgically resected specimens, and 5 autopsy specimens were studied. In the normal adult liver vascular smooth muscle cells and pericytes, together with perisinusoidal cells with thin cytoplasmic processes were positive for ASMA. These latter cells formed a loose and discontinuous layer along the sinusoidal walls. Immunoelectron microscopy showed that the ASMA-positive perisinusoidal cells were Ito cells containing fat droplets. The other sinusoidal lining cells were negative for ASMA. In chronic liver disease, ASMA-positive Ito cells showed an increase in number, size, and the intensity of immunostaining in areas of piecemeal necrosis), and formed a continuous cellular network. These cells were dendritic in shape with irregularly elongated cytoplasmic processes and contained an increased amount of microfilaments, in association with loss of the characteristic fat droplets. Thus, their ultrastructural features corresponded to those of myofibroblastic cells. Ito cells showed no staining for desmin in both normal and pathological livers. These results indicate that immunohistochemistry using an anti-ASMA antibody is a sensitive and reliable method for the identification of both normal and transformed Ito cells in adult human livers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194608
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sequential changes in human Ito cells and their relation to postnecrotic liver fibrosis in massive and submassive hepatic necrosis (1995)
    Springer
    Virchows Archiv 426 (1995), S. 95-101 
    Details
    ISSN: 1432-2307
    Keywords: Ito cell ; Fulminant hepatitis ; Postnecrotic fibrosis ; α-Smooth muscle actin ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To examine the relationship of Ito cells to postnecrotic liver fibrosis, liver specimens, obtained at autopsy from 17 patients with acute massive necrosis (AMN) and acute submassive hepatic necrosis (ASMN), were examined immunohistochemically. In normal adult livers, Ito cells positive for α-smooth muscle actin isoform (ASMA) were rarely seen, scattered along hepatic sinusoids. In contrast, in AMN the Ito cells in necrotic areas became strongly positive for ASMA. They were swollen with elongated cytoplasmic processes along collapsed sinusoidal walls. Around these ASMA-positive Ito cells, there were numerous infiltrated macrophages and lymphocytes present. There was no significant alteration of fibroblasts in the portal tracts. In the middle and late stages of ASMN, the spindle-shaped ASMA-positive Ito cells formed a continuous cellular network. New fibre formation was predominantly around them. In this immediate postnecrotic fibrosis, ASMA-positive stromal cells of Ito cell origin were distributed irregularly and were closely associated with reticulin and newly-formed collagen fibres. Regenerative nodules were surrounded by dense layers of ASMA-positive stromal cells. Throughout the stages of ASMN, portal fibroblasts remained negative for ASMA. We believe that Ito cells in necrotic areas show myofibroblastic transformation and play a central role in the postnecrotic liver fibrosis. Portal fibroblasts play no significant part in this type of fibrosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194703
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Estimation of distribution of vinyl groups in micron-sized monodisperse polymer microspheres by bromine titration method (1991)
    Springer
    Colloid & polymer science 269 (1991), S. 1126-1132 
    Details
    ISSN: 1435-1536
    Keywords: Polymeremulsion ; microsphere ; vinylgroup ; brominetitration ; dispersionpolymerization ; seededpolymerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The distribution of vinyl groups in polystyrene/poly(styrene-divinylbenzene) (PS/P(S-DVB)) composite microspheres produced by seeded copolymerization of S and DVB in the presence of 2.1-μm monodisperse PS seed particles was estimated from the amount detected with bromine titration method as a function of the reaction time under various conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00654120
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Preparation of micron-size monodisperse polymer microspheres having cationic groups (1992)
    Springer
    Colloid & polymer science 270 (1992), S. 733-737 
    Details
    ISSN: 1435-1536
    Keywords: Polymeremulsion ; microsphere ; chloromethylgroup ; polyamine ; cationicgroup ; dispersionpolymerization ; seededpolymerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Micron-size monodisperse crosslinked polymer microspheres having chloromethyl groups were prepared by seeded copolymerization of styrene, divinylbenzene, and chloromethylstyrene in the presence of 2.1-μ monodisperse polystyrene seed particles produced by dispersion polymerization. The modification reaction of chloromethyl groups on the surfaces with polyamines such as triethylenetetramine and ethylenediamine was carried out. From the measurements of ξ potential and the amount of chloride ion released, the introduction of a large number of cationic groups at the surfaces was confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00776143
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    Paper (German National Licenses)
    Fulltext
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