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  • 1
    Electronic Resource
    Electronic Resource
    GAX collagen in the treatment of urinary incontinence in elderly women: a two year follow up (1997)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 104 (1997), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective To determine the efficacy of GAX collagen in the treatment of elderly women with genuine stress incontinence.Design A single centre prospective study.Setting A London teaching hospital.Participants Thirty-four women older than 60 years of age with genuine stress incontinence diagnosed on videocystourethrography.Interventions Up to three paraurethral injections of GAX collagen.Main outcome measures Subjective and pad test assessments performed pre-operatively, after one month, one year and two years after the first injection of collagen. Videocystourethrography and urethral pressure profilometry were carried out pre-operatively, after three months and one year.Results There was a reduction in urinary leakage as measured on pad testing. After two years 77% of the surviving 26 women were symptomatically cured. Objectively after two years 48% of the women were cured and 9% were improved over their initial pre-treatment level of incontinence. The cure rate was the same whether or not the women had undergone previous vaginal surgery. The maximum urethral closure pressure was not increased with treatment indicating that collagen injections do not act by producing obstruction, but this should be interpreted with caution due to the small sample size.Conclusion GAX collagen is an effective method of treating urinary incontinence in the elderly and especially women who have undergone previous continence procedures. The technique is easy to perform and particularly suitable in those who are frail, but if failure occurs after two years consideration needs to be given to its cost effectiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1997.tb10657.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mapping of Malt Endopeptidase, NADH Diaphorase and Esterase Loci on Barley Chromosome 3L (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 112 (1994), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isoelectric focusing (IEF) and immunoblotting were used to detect genetic variants of malt endopeptidase (MEP1), an enzyme related to malting quality in barley and coded by the CepB locus on barley chromosome 3 (= 3H). A variant was found in a Turkish accession of wild barley (Hordeum vulgare ssp. spontaneum). The self progeny of a hybrid between this accession and the barley cultivar ‘Clipper’ were analyzed for recombination between the CepB locus and other isozyme loci. The estimates of recombination linked the CepB locus to an NADH diaphorase locus (Ndh2), which in turn was linked to the seedling esterase complex (Est1, Est2, and Est4) situated near the distal end of the long arm of chromosome 3. The Ndh2 locus was independent of two other NADH dehydrogenase loci (Ndh3, Ndh5) which were mapped on barley chromosome 5 (= 1H) in relation to the three hordein loci (Hor1, Hor2, and Hor3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1994.tb00685.x
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  • 3
    Electronic Resource
    Electronic Resource
    Does progesterone cause an irritable bladder? (1993)
    Springer
    International urogynecology journal 4 (1993), S. 259-261 
    Details
    ISSN: 1433-3023
    Keywords: Lower urinary tract ; Progesterone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Nine women with primary ovarian failure, who were having their artificial menstrual cycles manipulated with physiological levels of estrogen and high doses of progesterone, were entered into the study. They filled in urinary symptom questionnaires and had urodynamic investigations in the two phases of treatment: estrogen and progesterone, and estrogen alone. The number of voids per 24 hours was significantly greater in the progesterone phase, as was the end filling pressure on cystometry. This is the first report in the literature of the effects of high-dose progesterone on the lower urinary tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00372731
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of combining scald resistance genes on disease levels, yield and quality traits in barley (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 361-366 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pyramiding  ;  Hordeum vulgare ssp. spontaneum  ;  Rhynchosporium secalis  ;  Backcross lines  ;   Isozyme marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pairwise combinations of genes for resistance to scald in barley were developed using linked isozyme markers to test whether such combinations conferred improved resistance to the pathogen, Rhynchosporium secalis. The resistance genes originally derived from Hordeum vulgare ssp. spontaneum. The combinations were bred into an essentially similar genetic background because the scald-susceptible, Australian barley cultivar ‘Clipper’ was the recurrent backcross parent in their ancestry. In field tests of the recombinants over 2 years, disease levels were lower in three of six doubly resistant lines than in backcross lines carrying a single resistance gene, which in turn were less diseased than either ‘Clipper’ or recombinants that lacked the marked resistance genes. All resistant lines significantly outyielded ‘Clipper’ but did not themselves differ significantly. Lines resistant to scald had significantly higher grain size and grain weight. Gains for malt yield of about 1% were detected in the higher disease environment. Resistance was not accompanied by any obvious “cost” in terms of yield or quality. Protection against scald is therefore a significant requirement for new malting barley cultivars in scald-prone areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050289
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of combining scald resistance genes on disease levels, yield and quality traits in barley (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 361-366 
    Details
    ISSN: 1432-2242
    Keywords: Pyramiding ; Hordeum vulgare ssp ; spontaneum ; Rhynchosporium secalis ; Backcross lines ; Isozyme marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pairwise combinations of genes for resistance to scald in barley were developed using linked isozyme markers to test whether such combinations conferred improved resistance to the pathogen, Rhynchosporium secalis. The resistance genes originally derived from Hordeum vulgare ssp. spontaneum. The combinations were bred into an essentially similar genetic background because the scald-susceptible, Australian barley cultivar ‘Clipper’ was the recurrent backcross parent in their ancestry. In field tests of the recombinants over 2 years, disease levels were lower in three of six doubly resistant lines than in backcross lines carrying a single resistance gene, which in turn were less diseased than either ‘Clipper’ or recombinants that lacked the marked resistance genes. All resistant lines significantly outyielded ‘Clipper’ but did not themselves differ significantly. Lines resistant to scald had significantly higher grain size and grain weight. Gains for malt yield of about 1 % were detected in the higher disease environment. Resistance was not accompanied by any obvious “cost” in terms of yield or quality. Protection against scald is therefore a significant requirement for new malting barley cultivars in scald-prone areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223177
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  • 6
    Electronic Resource
    Electronic Resource
    Characterisation of a gene encoding wheat endosperm starch branching enzyme-I (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 156-163 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum tauschii ; Starch branching enzyme genes ; Wheat ; Endosperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A genomic DNA fragment from Triticum tauschii, the donor of the wheat D genome, contains a starch branching enzyme-I (SBE-I) gene spread over 6.5 kb. This gene (designated wSBE I-D4) encodes an amino acid sequence identical to that determined for the N-terminus of SBE-I from the hexaploid wheat (T. aestivum) endosperm. Cognate cDNA sequences for wSBE I-D4 were isolated from hexaploid wheat by hybridisation screening from an endosperm library and also by PCR. A contiguous sequence (D4 cDNA) was assembled from the sequence of five overlapping partial cDNAs which spanned wSBE I-D4. D4 cDNA encodes a mature polypeptide of 87 kDa that shows 90% identity to SBE-I amino acid sequences from rice and maize and contains all the residues considered essential for activity. D4 mRNA has been detected only in the endosperm and is at a maximum concentration mid-way through grain development. The wSBE I-D4 gene consists of 14 exons, similar to the structure for the equivalent gene in rice; the rice gene has a strikingly longer intron 2. The 3′ end of wSBE I-D4 was used to show that the gene is located on group 7 chromosomes. The sequence upstream of wSBE I-D4 was analysed with respect to conserved motifs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051052
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  • 7
    Electronic Resource
    Electronic Resource
    Genes for scald resistance from wild barley (Hordeum vulgare ssp spontaneum) and their linkage to isozyme markers (1991)
    Springer
    Euphytica 61 (1991), S. 225-231 
    Details
    ISSN: 1573-5060
    Keywords: allozyme markers ; barley ; genetics of resistance ; Hordeum vulgare ; Rhynchosporium secalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Accessions of Hordeum vulgare ssp. spontaneum, the wild progenitor of barley, collected in Israel (70), Iran (15) and Turkey (6) were screened for seedling response to four isolates of Rhynchosporium secalis, the pathogen causing leaf scald in barley. Resistance was very common in the collection (77%) particularly among accessions from the more mesic sites (90%). The genetics of this resistance were investigated in fifteen backcross (BC3) lines that contained an isozyme variant from H.v. ssp. spontaneum in a H.v. ssp. vulgare (cv. Clipper) background and were resistant to scald. Segregation in the BC3F2 families conformed with a single dominant resistance gene in 9 of the 15 lines. Scald resistance and the isozyme marker were closely linked in three of the BC3-lines, loosely linked in four and unlinked in the remaining eight. Scald resistance genes were identified on barley chromosomes 1, 3, 4 and 6. Crosses between several of the scald resistant BC-lines together with the linkage data indicated that at least five genetically independent resistances are available for combining together for deployment in barley. The linkage of scald resistance in several BC3-lines to the isozyme locus Acp2 is of special interest as this locus is highly polymorphic in wild barley.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039662
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