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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 77 (1995), S. 4616-4622 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Sm-doped ZnS thin films were grown by metalorganic chemical vapor deposition using diethyl- zinc, H2S, Sm(thd)3 (thd=2,2,6,6-tetramethyl-3,5-heptanedione), Sm(hfa)3 (hfa=1,1,1,5,5,5- hexafluoro-2,4-pentanedione), SmCl3, and HCl. The following films were prepared: an undoped ZnS, a ZnS:Sm grown with Sm(thd)3 and without HCl, a ZnS:Sm,Cl grown with both Sm(thd)3 and HCl, a ZnS:Cl grown only with HCl, and a ZnS:SmCl3 grown with SmCl3. Very little Sm was incorporated when Sm(hfa)3 was used as the dopant source. In the ZnS:Sm, the Sm was not segregated at grain boundaries, at least at concentrations below 0.2 at. %. In the ZnS:Sm and the ZnS:Sm,Cl, the (200), (220), and (311) reflections of the cubic structure that the undoped ZnS and the ZnS:Cl exhibited were not detected, and preference for the (00⋅1) hexagonal orientation became stronger. The electroluminescence spectrum of the ZnS:Sm,Cl showed three satellite peaks appearing in each of the 7 nm ranges around the three dominant peaks seen in the ZnS:Sm. The total electroluminescence intensity of the ZnS:Sm,Cl increased as a result of the appearance of the satellite peaks. While the ZnS:Sm,Cl showed photoluminescence attributed to the self-activated centers, its intensity was less than 10−2 of that of the ZnS:Cl. A model is suggested that Sm-VZn (zinc vacancy)-Cl complex centers are formed by the Cl codoping and give rise to the satellite peaks. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: ZnS:Sm, ZnS:Sm,Cl, and ZnS:Cl thin films were grown by metalorganic chemical vapor deposition using the thd-chelate (thd=2,2,6,6-tetramethyl-3,5-heptanedione) of Sm and hydrogen chloride. The electroluminescence spectrum of the ZnS:Sm,Cl showed three satellite peaks appearing in each of the 7 nm ranges around the three dominant peaks that the ZnS:Sm has originally. The ZnS:Sm,Cl also showed photoluminescence attributed to the self-activated centers, but its intensity was less than 10−2 of that of the ZnS:Cl. The effect of the Cl codoping on Sm3+ luminescent properties is discussed on the basis of the Sm-VZn (zinc vacancy)-Cl complex formation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 81 (1997), S. 445-450 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: ZnS:Tm and ZnS:Tm,Cl thin films were grown by metalorganic chemical vapor deposition (MOCVD), using diethylzinc, H2S, Tm(thd)3 (thd=2,2,6,6-tetramethyl-3,5-heptanedione), and HCl. The ZnS:Tm did not contain oxygen which might be introduced through the thd-radical. It thus has only codopant-free Tm3+ luminescent centers probably associated with native defects. The electroluminescence (EL) spectrum of the ZnS:Tm,Cl showed three satellite emission lines in addition to the original emission of the ZnS:Tm, indicating the existence of Tm–Cl complex centers. In contrast, the photoluminescence (PL) spectrum of the ZnS:Tm,Cl under host excitation showed no discernible satellite emission lines. Hence, though the Tm ions in the Tm–Cl complex centers are expected to be charge compensated by Cl or a certain Cl-induced defect, they are rather inactive in the PL excitation while active in the EL excitation. The same properties were observed for the MOCVD-grown ZnS:Sm and ZnS:Sm,Cl [A. Kato, M. Katayama, A. Mizutani, N. Ito, and T. Hattori, J. Appl. Phys. 77, 4616 (1995)], and therefore they probably occur for other rare-earth luminescent centers with Cl codopant. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Melbourne, Australia : Blackwell Science Pty
    International journal of urology 6 (1999), S. 0 
    ISSN: 1442-2042
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Background : The hematoma size relative to the body size was measured on computed tomography films using a personal computer system in order to define whether that parameter is useful for decision-making in the management of blunt renal trauma. Methods : From 1982 to 1997, 33 patients with intermediate or severe grade blunt renal trauma were retrospectively divided into three groups: group 1, managed conservatively without transcatheter embolization; group 2, managed by bedrest after selective transcatheter embolization; and group 3, managed operatively. In these three groups, the hematoma area (H) and the ratio of hematoma area to body area on CT (H/B) were measured and the chronological changes of the H/B in groups 1 and 2 were studied. Results : The H and H/B of group 3 were significantly larger than those of group 1. The H/B was more clearly distinguished for each group compared with the H alone. Well-preserved kidney integrity, despite the presence of a large hematoma in group 2, allowed the conservative treatment following transcatheter embolization of the bleeding site. The H/B of all group 1 patients gradually decreased and on the 40th or 50th day after injury they reached a level equivalent to the ratio of contra-lateral normal kidney area to body area. Conclusion : The ratio of hematoma area to body area on CT was very useful in evaluating the grade of blunt renal trauma. In conservative treatment for blunt renal trauma changes of the hematoma size is a useful indicator for management.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1438-8359
    Keywords: Double-lumen tube ; Complications ; Tube troubles ; Airway obstruction ; One-lung ventilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose Several case reports indicate critical respiratory complications in relation to the double-lumen endobronchial tube (DLT). A prospective survey for the airway problems in using the DLT is presented. Methods One hundred adult patients undergoing thoracotomy for lung cancer were investigated. Tube malposition and airway obstruction were searched using a fiber-optic scope. The endobronchial cuff was positioned just below the trachcal carina while the trachea was intubated with a DLT (Rüsch). The distances of displacement, from the tracheal carina to the bronchial cuff, were measured during anesthesia using an epidural catheter, which had marks every 5 mm. The distances for correcting the tube position were measured at both the bronchial cuff and the level of the teethPaO2,PaCO2 andSPO2 were also measured. Results Malposition (displacement over 5 mm from the correct position) was found in 42 patients, and 40 of them were in a withdrawal direction, occurring at the postural change and during one-lung ventilation, especially during manipulation of the lung hilum. Correcting distances at the level of the teeth were 15.3–3-times longer than those at the bronchial cuff. Airway deformities and gradual withdrawal of the bronchial cuff were found in association with surgical manipulation. Obstruction occurred at the tips of the tracheal tube in four patients and the bronchial tube in six patients, and at the tip of both in two patients. Hypoxemia (PaO2〈60 mmHg) occurred in four patients and hypercapnea (PaCO2〉60 mm Hg) in two patients. Conclusion Most of the DLT obstructions were associated with withdrawal malposition. Great attention to DLT displacement and airway deformity is advised.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1084
    Keywords: CT ; Liver cirrhosis ; Pancreas ; Trophic effect ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the effect of hepatic dysfunction on pancreatic morphology, the antero-posterior width of the pancreas was measured by CT in 50 non-alcoholic cirrhotic patients without pancreatic disease and 221 age-and sex-matched normal controls. In addition, we reviewed the histology of the pancreas from 13 autopsies of the cirrhotics and 13 controls. The mean width of the pancreatic head in the cirrhotics was 2.94 ∓ 0.34 cm (mean ∓ SD), which was significantly larger than that in the controls (2.17 ∓ 0.32 cm) (P 〈 0.001). The width of the pancreatic body in the cirrhotics was also significantly greater than in the controls (1.95 ∓ 0.24 cm versus 1.50 ∓ 0.30 cm) (P 〈 0.001). Histological investigation revealed that lipomatosis and fibrosis increased with age in the controls, while they were seldom observed in the cirrhotics. And enlargement of acinar cells or of the islets of Langerhans was often seen in the cirrhotics. These data suggested that advanced liver dysfunction provoked pancreatic growth, probably through a trophic effect.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 31 (1996), S. 843-852 
    ISSN: 1573-5028
    Keywords: glyoxysome ; leaf peroxisome ; microbody transition ; pumpkin (Cucurbita sp.) ; senescence ; 3-ketoacyl-CoA thiolase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone for 3-ketoacyl-CoA thiolase (EC 2.3.1.16) was isolated from a λgt11 cDNA library constructed from the poly(A)+ RNA of etiolated pumpkin cotyledons. The cDNA insert contained 1682 nucleotides and encoded 461 amino acid residues. A study of the expression in vitro of the cDNA and analysis of the amino-terminal sequence of the protein indicated that pumpkin thiolase is synthesized as a precursor which has a cleavable amino-terminal presequence of 33 amino acids. The amino-terminal presequence was highly homologous to typical amino-terminal signals that target proteins to microbodies. Immunoblot analysis showed that the amount of thiolase increased markedly during germination but decreased dramatically during the light-inducible transition of microbodies from glyoxysomes to leaf peroxisomes. By contrast, the amount of mRNA increased temporarily during the early stage of germination. In senescing cotyledons, the levels of the thiolase mRNA and protein increased again with the reverse transition of microbodies from leaf peroxisomes to glyoxysomes, but the pattern of accumulation of the protein was slightly different from that of malate synthase. These results indicate that expression of the thiolase is regulated in a similar manner to that of other glyoxysomal enzymes, such as malate synthase and citrate synthase, during seed germination and post-germination growth. By contrast, during senescence, expression of the thiolase is regulated in a different manner from that of other glyoxysomal enzymes.
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  • 8
    ISSN: 1573-5028
    Keywords: β-glucuronidase gene ; endosperm-specific expression ; 5′ deletions ; glutelin gene ; rice ; transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5′ upstream region of the rice storage protein type II glutelin gene was examined for its regulatory function in transgenic tobacco. Chimeric genes containing 5′ flanking regions of the glutelin gene transcriptionally fused to the β-glucuronidase (GUS) reporter gene were introduced into the tobacco genome by Agrobacterium tumefaciens-mediated gene transfer. The chimeric genes were expressed specifically in developing seeds, as opposed to leaves and stems, of the transgenic tobacco. Histochemical analysis revealed that the GUS activity was restricted to the endosperm tissue. A deletion series of the 5′ flanking region was created from position -1329 to -74 relative to the transcriptional initiation site and similarly examined in transgenic tobacco. Measurement of GUS activity of the seeds from the transgenic plants bearing the chimeric genes indicated that the region between positions -441 and -237 was required for the temporal and endosperm-specific expression of the GUS activity in tobacco. RNA analysis by northern blotting confirmed the importance of the -441 to -237 region. Addition of up to 888 bp to the -441 deletion resulted in little increase in GUS activity, although all constructs expressing the GUS gene showed a similar tissue and temporal regulation pattern.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 17 (1991), S. 875-885 
    ISSN: 1573-5028
    Keywords: glutelin ; multigene family ; Oriza sativa ; seed storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the split between the two varieties. While corresponding exons of the subfamily B showed 80 to 88% nucleotide sequence homology, those exons were only 60–65% homologous to those of the glutelin A subfamily [15, 19, 24], distinguishing them from the subfamily A. Intron position and derived polypeptide structure, in addition to the nucleotide sequence, confirm the subfamily B members as glutelins. Analysis of RNA from seeds of different stages of development showed that the subfamily B members were expressed at the same time as those of subfamily A, demonstrating coordinated regulation of the two subfamilies.
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  • 10
    ISSN: 1573-5028
    Keywords: amino-terminal presequence ; citrate synthase ; glyoxysome ; leaf peroxisome ; microbody transition ; pumpkin (Cucurbita sp.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone for glyoxysomal citrate synthase (gCS) was isolated from a λgt11 cDNA library prepared from etiolated pumpkin cotyledons. The cDNA of 1989 bp consisted of a 1548 bp open reading frame that encoded 516 amino acid residues. The deduced amino acid sequence of gCS did not have a typical peroxisomal targeting signal at its carboxyl terminal. A study of expression in vitro of the cDNA and an analysis of the amino-terminal sequence of the citrate synthase indicated that gCS is synthesized as a larger precursor that has a cleavable amino-terminal presequence of 43 amino acids. The predicted amino-terminal sequence of pumpkin gCS was highly homologous to those of other microbody enzymes, such as 3-ketoacyl-CoA thiolase of rat and malate dehydrogenase of watermelon that are also synthesized as precursors of higher molecular mass. Immunoblot analysis showed that the level of gCS protein increased markedly during germination and decreased rapidly during the light-induced transition of microbodies from glyoxysomes to leaf peroxisomes. By contrast, the level of mRNA for gCS reached a maximum earlier than that of the protein and declined even in darkness. The level of the mRNA was low during the microbody transition. These results indicate that the accumulation of the gCS protein does not correspond to that of the mRNA and that degradation of gCS is induced during the microbody transition, suggesting that post-transcriptional regulation plays an important role in the microbody transition.
    Type of Medium: Electronic Resource
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