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  • 1
    Electronic Resource
    Electronic Resource
    A 300 kilobase interval genetic map of rice including 883 expressed sequences (1994)
    [s.l.] : Nature Publishing Group
    Nature genetics 8 (1994), S. 365-372 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] We have constructed a high resolution rice genetic map containing 1,383 DNA markers at an average interval of 300 kilobases (kb). The markers, distributed along 1,575 cM on 12 linkage groups, comprise 883 cDNAs, 265 genomic DNAs, 147 randomly amplified polymorphic DNAs (RAPD) and 88 other DNAs. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1294-365
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  • 2
    Electronic Resource
    Electronic Resource
    Affinity of bone sialoprotein and several other bone and dentin acidic proteins to collagen fibrils (1992)
    Springer
    Calcified tissue international 51 (1992), S. 438-442 
    Details
    ISSN: 1432-0827
    Keywords: Bone sialoprotein ; Phosphophoryn ; Bone Gla protein ; Bone proteoglycan ; Collagen binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Bone and dentin contain several kinds of mineral-binding proteins and cell-attachment proteins. The authors examined the affinity of these proteins to type I collagen, a major matrix protein of the tissue. Bone sialoprotein (BSP), bone Gla protein (BGP), bone small proteoglycan II (PG II), osteonectin (ON), and dentin phosphophoryn (DPP) were labeled with fluorescein isothiocyanate and incubated with reconstituted type I collagen fibril. DPP, BGP, BSP, and PG II were absorbed significantly to the collagen fibril at physiological ionic strength with dissociation constants of 10-6–10-7 M. BSP and PG II enhanced the fibrillogenesis of collagen. These acidic proteins can affect the surface properties of collagen fibril, and BSP, having the cell-attachment sequence Arg-Gly-Asp, possibly mediates interaction between collagen fibril and cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296677
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  • 3
    Electronic Resource
    Electronic Resource
    Extracellular processing of dentin matrix protein in the mineralizing odontoblast culture (1995)
    Springer
    Calcified tissue international 57 (1995), S. 237-241 
    Details
    ISSN: 1432-0827
    Keywords: Odontoblast ; Dentin ; Mineralization ; Casein kinase II ; Phosphophoryn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Odontoblasts that we prepared from bovine incisors produced a dentin-specific protein, phosphophoryn, and accumulated it in mineralized nodules. The time course of mineralization was detected by measuring osteocalcin and mineral in the nodules. The sequence of developmental expression of proteins in this mineralizing dentin cell culture is very similar to that in bone cells, suggesting a common mechanism for matrix mineralization in bone and dentin. Casein kinase II, which phosphorylates bone phosphoproteins and dentin phosphophoryn, also emerges coinciding with the initiation of mineralization. Furthermore, we have detected extracellular phosphorylation by casein kinase II of a dentin protein of Mr 60,000, which we recovered from the phosphophoryn fraction in CaCl2 precipitate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310265
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  • 4
    Electronic Resource
    Electronic Resource
    Bone Chondroadherin Promotes Attachment of Osteoblastic Cells to Solid-State Substrates and Shows Affinity to Collagen (1996)
    Springer
    Calcified tissue international 59 (1996), S. 163-167 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Chondroadherin — Cell attachment — Collagen.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Chondroadherin, which is reported to be synthesized by chondrocytes and to promote their attachment, was purified from bovine bone. It was a minor component of bone organic matrix, and was present in the 4 M guanidine extract of demineralized bone. Chondroadherin promoted attachment of osteoblastic cells to solid-state substrates, and bound to collagen. Binding of chondroadherin to collagen was significantly higher than that of osteonectin or decorin. These findings imply that chondroadherin may play a role in maintaining bone cells on the collagen matrices of bone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900103
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  • 5
    Electronic Resource
    Electronic Resource
    Further characterization of interaction between bone sialoprotein (BSP) and collagen (1995)
    Springer
    Calcified tissue international 56 (1995), S. 140-144 
    Details
    ISSN: 1432-0827
    Keywords: Bone sialoprotein ; Collagen ; Hole zone ; Calcification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Bone sialoprotein (BSP) has an affinity to collagen fibrils [25]. A role of carbohydrate chains in the affinity was examined by removing sialic acids of BSP. Neuraminidase treatment of the BSP increased the binding to collagen. Binding sites of BSP on collagen were examined by biochemical and electron-microscopic methods. Purified bovine BSP was labeled with biotin. Collagen α chains or CNBr peptides were separated by electrophoresis and transfered to nitrocellulose membranes. The membranes were incubated with the biotin-labeled BSP, and the bound BSP was visualized with avidin conjugated with alkaline phosphatase. The labeled BSP was preferentially bound to the α 2 chain, and peptides derived from α 2 chain. In another experiment, the labeled BSP was incubated with reconstituted native collagen fibrils. The mixture was put on a copper grid, reacted with avidin conjugated with gold particles, and observed with an electron microscope. The gold particles were seen mainly within hole zones of the fibrils. BSP bound to the α 2 chain within the hole zones may regulate the onset of calcification at hole zones and the cell binding to collagen fibrils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296346
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  • 6
    Electronic Resource
    Electronic Resource
    Conformational changes of bovine bone osteonectin induced by interaction with calcium (1995)
    Springer
    Calcified tissue international 56 (1995), S. 559-565 
    Details
    ISSN: 1432-0827
    Keywords: Osteonectin ; Calcium binding ; Calcium-induced conformational change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract To clarify calcium-induced conformational changes in bovine bone osteonectin, the protein was labeled with fluorescein isothiocyanate (FITC) in the presence and absence of calcium. By calcium titration using fluorescence spectrometry, it was demonstrated that FITC-osteonectin labeled in the presence of 2 mM CaCl2 showed a much higher affinity for calcium ions than did that labeled in the absence of calcium ions. The midpoint for completion of the increase in the intrinsic fluorescence (K0.5) of the two were 1×10-7 M and 5×10-7 M, respectively. By tryptic digestion and isolation of the fluorescent peptide of both FITC-osteonectins, the site of FITC-labeling was determined to be Lys174. Furthermore, it was found that the efficacy of labeling in this specific binding site was three times higher in the FITC-osteonectin labeled in the presence of 2 mM CaCl2 than in that labeled in the absence of calcium. The results indicate that in the presence of 2 mM CaCl2 the microenvironment around Lys174 of osteonectin was more open to modification than in the absence of calcium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298590
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  • 7
    Electronic Resource
    Electronic Resource
    Stimulation by bone sialoprotein of calcification in osteoblast-like MC3T3-E1 cells (1995)
    Springer
    Calcified tissue international 56 (1995), S. 403-407 
    Details
    ISSN: 1432-0827
    Keywords: Bone sialoprotein ; Fibronectin ; Type I colfagen ; MC3T3-E1 cells ; Calcification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Bone sialoprotein (BSP) containing an Arg-Gly-Asp cell-binding sequence was purified from bovine bone 4 M guanidine-HCl extract after HCl demineralization by a series of chromatographic procedures. When this protein was coated on culture dishes in the presence of type I collagen, it increased both DNA content and alkaline phosphatase (ALP) activity in osteoblast-like MC3T3-E1 cells, and stimulated calcification in the cells, whereas fibronectin, another cell-binding protein, showed a marked increase in the DNA content but had little effect on the ALP activity. These findings suggest that BSP is mitogenic for preosteoblasts and differentiating the cells into osteoblasts, thereby stimulating bone calcification
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301610
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  • 8
    Electronic Resource
    Electronic Resource
    Biochemical properties of collagen from ligaments and periarticular tendons of the human knee (1994)
    Springer
    Knee surgery, sports traumatology, arthroscopy 2 (1994), S. 229-233 
    Details
    ISSN: 1433-7347
    Keywords: Collagen ; Ligaments ; Periarticular tendons ; Human knee
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Sports Science
    Notes: Abstract The biochemical properties of collagens from the periarticular connective tissues of the human knee (ligaments, semitendinosus and gracilis tendons, and the iliotibial band) were investigated in subjects ranging from 20 to 70 years of age. Although the total collagen content of most tissues was more than 90% of the dry weight, the anterior cruciate ligament and the iliotibial band had relatively low collagen contents. There were no significant changes in the tissue collagen content with aging. However, the anterior cruciate ligament and the patellar tendon of the 20-year-old subject had a higher content of soluble collagen than the other ligaments and tendons. The iliotibial band of the 20-year-old subject contained more collagen that was soluble by a combination of salt, sodium citrate extractions and pepsin digestion. Dihydroxylysinonorleucine was the major reducible cross-link of collagen from all the ligaments. The amount of dihydroxylysinonorleucine in the anterior cruciate ligament of the 20-year-old subject was much higher than that in the other ligaments. In contrast, the tendons and the iliotibial band contained a large amount of histidino-hydroxymerodesmosine and hydroxylysinonorleucine, while the patellar tendon was the only tendon with a significant content of dihydroxylysinonorleucine. Hydroxypyridinium non-reducible cross-links were more abundant in collagens from ligaments than in collagens from the other tissues. The cross-link study and the analysis of collagen solubility showed that patellar tendon collagen more closely resembled the collagen from the anterior cruciate ligament than that from periarticular tendons. It was also shown that the anterior cruciate ligament contains relatively immature collagen compared with the other ligaments. Our findings may contribute to an improved understanding of the functions of the ligaments and tendons around the knee joint. In addition, the data may provide some useful pointers that will help in selection of periarticular tissues as appropriate substitutes for ligament replacement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01845593
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  • 9
    Electronic Resource
    Electronic Resource
    Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 37-44 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; RFLP mapping ; QTL ; Heading date ; Backcross progeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Fine mapping was carried out on three putative QTLs (tentatively designated as Hd-1 to Hd-3) of five such QTLs controlling heading date in rice that had been earlier identified using an F2 population derived from a cross between a japonica variety, ‘Nipponbare’, and an indica variety, ‘Kasalath’, using progeny backcrossed with ‘Nipponbare’ as the recurrent parent. One BC3F2 and two BC3F1 plants, in which the target QTL regions were heterozygous and most other chromosomal regions were homozygous for the ‘Nipponbare’ allele, were selected as the experimental material. Self-pollinated progeny (BC3F2 and BC3F3) of the BC3F1 or BC3F2 showed continuous variation in days to heading. By means of progeny testing based on BC3F3 or BC3F4 lines, we determined the genotypes of each BC3F2 or BC3F3 individual at target QTLs. Their segregation patterns fitted Mendelian inheritance ratios. When the results obtained by RFLP analysis and progeny tests were combined, Hd-1, Hd-2 and Hd-3 were mapped precisely on chromosomes 6, 7 and 6, respectively, of a rice RFLP linkage map. The results demonstrated that QTLs can be treated as Mendelian factors. Moreover, these precise locations were in good agreement with the regions estimated by QTL analysis of the initial F2 population, demonstrating the high reliability of QTL mapping using a high-density linkage map.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050864
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  • 10
    Electronic Resource
    Electronic Resource
    BMP-Induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: Topology of osteogenesis (1998)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 39 (1998), S. 190-199 
    Details
    ISSN: 0021-9304
    Keywords: BMP ; geometry ; carriers ; hydroxyapatite ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Bone morphogenetic protein (BMP) is known to require a suitable carrier to induce ectopic bone formation in vivo. Hydroxyapatite ceramics have been reported to be effective in some forms but ineffective in others as a carrier of BMP-induced bone formation. In this study we compare three geometrically different forms of hydroxyapatite to examine their functions as carriers of BMP-induced bone formation. A fraction containing all the active BMPs (BMP cocktail) was partially purified from a 4M guanidine extract from bovine bone by a three-step chromatographic procedure. The BMP cocktail was combined with each of three forms of hydroxyapatite - solid particles (SPHAP), porous particles (PPHAP), and coral-replicated porous tablets (coral-HAP) - and implanted subcutaneously into rats. Both the PPHAP and coral-HAP systems induced osteogenesis 2 weeks after implantation, as evidenced by morphological and biochemical observations. Details of the osteogenetic process were followed by double-fluorescence labeling in the coral-HAP system to confirm bone formation on the surface of hydroxyapatite. However, there was no evidence of osteogenesis or chondrogenesis in the SPHAP system. The results indicate that the geometry of the interconnected porous structure in PPHAP and coral-HAP create spaces for vasculature that lead to osteogenesis while the smooth structure and close contact of particles in SPHAP inhibit vascular formation and proliferation of mesenchymal cells, preventing bone and cartilage formation. It was concluded that the geometrical structure in hydroxyapatite ceramics that induces vasculature is crucial as a carrier for BMP-induced bone formation. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 190-199, 1998.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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