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Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography (1996)

Inoue, H. ; Nonomura, Y. ; Ashizawa, A. ; [et al.]

Springer

Chromatographia 43 (1996), S. 361-364

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Bacteriochlorophylla ; Copper(II) bacteriochlorophylla ; Zinc(II) bacteriochlorophylla

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophylla [Cu(II)-BChl-a] and zinc(II) bacteriochlorophylla [Zn(II)-BChl-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25∶75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02271011

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Effect of organic solvents on the activity of glucoamylase (1986)

Nagamoto, H. ; Yasuda, T. ; Inoue, H.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 28 (1986), S. 1172-1177

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Hydrolyses of maltose, maltotriose, and soluble starch catalyzed by glucoamylase (Asp. Niger) were carried out in the aqueous solutions of methanol, ethanol, ethylene glycol, and 1,4-dioxane at 40°C and at the optimum pH in the respective solutions. By the kinetic analysis based on the subsite model, it was shown that the intrinsic rate constant, Kint, was electrostatically affected by the dielectric constant of the hydroorganic solutions. The affinity of the third subsite, A3, which affects the apparent rate constant, K0, was correlated with the ×Gtr's of maltose and amino acid side chains.

Additional Material: 5 Ill.