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  • 1995-1999  (2)
  • 1985-1989
  • 1935-1939
  • Klebsiella  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Der Pathologe 19 (1998), S. 384-387 
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Rhinosklerom ; Plasmozytom ; Eindonasaler Tumor ; Klebsiellen ; Mikulicz-Zellen ; Key words Rhinoscleroma ; Plasmocytoma ; Endonasal tumour ; Klebsiella ; Mikulicz cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Histologic, immunohistochemical and ultrastructural results of a rhinoscleroma diagnosed bioptically on a 38 aged man are demonstrated. The characteristic light microscopic sign is a plasmocytic inflammatory infiltrate with dense packed foamy transformed macrophages (Mikulicz cells) ultrastructurally containing diplobacilli (Klebsiella rhinoscleromatis, gram negative agent of the lesion). The lesion has to be differentiated as of other inflammatory diseases as of plasmocytoma. A local application of acriflavin solution 2% was of therapeutical benefit.
    Notes: Zusammenfassung Vorgestellt wird die Pathologie der Rhinoskleroms am Beispiel eines 38 Jahre alten Mannes in Verbindung mit klinischen Befunden. Dieser im bioptischen Eingangsgut selten beobachtete, klinisch tumorartig imponierende Prozeß weist ein charakteristisches plasmazellreiches entzündliches Infiltrat auf, das rasenförmige Schaumzellnester (sog. Mikulicz-Zellen) einschließt, in denen ultrastrukturell der Erreger (Klebsiella rhinoscleromatis) nachgewiesen werden kann, der die Diagnose morphologisch allein sichert. Eine Lokalbehandlung mit 2%iger Acriflavin-Lösung führte zu einer deutlichen klinischen Remission.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: Key words Cyclodextrin metabolism ; Klebsiella ; oxytoca ; Cyclodextrinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It has been shown previously that the products of 11 genes are required for metabolism of starch by Klebsiella oxytoca via a novel pathway. An extracellular cyclodextrin glucanotransferase first degrades starch into α- and β-cyclodextrins; evidence then has been presented that the cyclodextrins are transported into the cytoplasma via a specific system and that they are metabolised inside the cell. To provide support for this model, we have analysed whether Klebsiella oxytoca possesses a cytoplasmic enzyme able to linearise cyclodextrins. A possible candidate was the product of the cymH gene since it displays sequence similarity with cyclodextrinases from other organisms. The cymH gene was overexpressed, and the CymH protein was purified. CymH is a monomer of 69 kDa molecular mass and hydrolysed cyclodextrins at an optimum pH of 7.0 and an optimum temperature of 23° C, respectively. The apparent K m increased with increasing size of the cyclodextrins, but the reaction velocity decreased. Linear malto-oligosaccharides were also accepted as substrates, but were hydrolysed with a lower efficiency. Final products in each case were maltose and maltotriose. It was demonstrated by immunoblotting that CymH is located in the cytoplasm and that no signal peptide was cleaved off. Since cymH mutants were no longer able to grow on cyclodextrins, these results prove that cyclodextrins are degraded inside the cell, and they support the contention of the existence of a specific transport system.
    Type of Medium: Electronic Resource
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