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  • 1
    Electronic Resource
    Electronic Resource
    Effect of human serum on alkaline phosphatase induction in cultured human tumor cells (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 753-755 
    Details
    ISSN: 1420-9071
    Keywords: Alkaline phosphatase ; human serum ; cultured tumor cells ; enzyme induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The continuous cell lines T 24 and HT-29, derived from human bladder and colon carcinomas, produce term-placental and intestinal alkaline phosphatase, respectively. Growth in hyperosmolar medium or exposure to prednisolone or sodium butyrate induces increased enzyme levels, and combinations of inducers elicit synergistic activity increases. The effect of the inducing agents is strikingly diminished when cells are grown in the presence, of high concentrations of human serum, and the synergistic increases are essentially abolished. Major human serum protein fractions do not affect alkaline phosphatase induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974578
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The use of 5-fluorocytosine and ketoconazole in the culture of the erythrocytic stages ofPlasmodium falciparum and some tumor cell lines (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 478-480 
    Details
    ISSN: 1420-9071
    Keywords: 5-Fluorocytosine ; ketoconazole ; malaria ; tumor cell ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In vitro culture systems are often contaminated by bacteria and fungi. It is therefore often necessary to supplement culture media with agents such as penicillin/streptomycin, gentamycin or amphotericin B. The latter cannot be used in the in vitro culture of erythrocytic stages ofP. falciparum, and thus anti-fungal agents have not been regularly used in this system. We describe the prophylactic use of 5-fluorocytosine (5-FC) and ketoconazole (KTZ) in tissue cultures at concentrations up to 300 and 10 μg/ml respectively which have no effect on the growth ofP. falciparum (FCR-3 strain). A melanoma cell line (C32) and a line of uterine carcinoma (C41) were also unaffected by similar concentrations of 5-FC and KTZ. When dissolved in complete culture medium (RPMI 1640) with 10% human plasma, the minimum inhibitory concentration of 5-FC for a susceptible strain ofCandida remained below 2 μg/ml. These experiments suggest that 5-FC (at 50 μg/ml) alone or in combination with KTZ (at 1 μg/ml) is a useful addition to the armamentarium of antimicrobials available to the tissue culture biologist for a variety of cell culture systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952037
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Berechnung einer stattgehabten Entrahmung auf Grund der sogenannten Stallprobe (1919)
    Springer
    European food research and technology 38 (1919), S. 100-101 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02037897
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Stress-response (heat-shock) protein 90 expression in tumors of the central nervous system: an immunohistochemical study (1995)
    Springer
    Acta neuropathologica 89 (1995), S. 184-188 
    Details
    ISSN: 1432-0533
    Keywords: Key words Stress-response protein 90 ; Heat-shock ; protein 90 ; Brain tumors ; Meningiomas ; Breast tumor metastases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This retrospective study deals with the expression of stress-response (heat-shock) protein 90 (srp 90) in a series of 148 human brain tumors. Immunohistochemical procedures were employed; cells of the human breast cancer line MCF 7 exposed to hyperosmolar stress served as positive controls. Deposits of reaction products were found in the cytoplasm and they displayed a granular pattern. srp 90 was detected in 14/31 meningiomas and 5/10 breast cancer metastases to the brain. The protein was also present in 6/13 glioblastomas and 7/18 astrocytomas. In addition, a positive reaction was found in 2/10 medulloblastomas, 2/14 primitive neuroectodermal tumors, 1/11 pituitary tumor, 2/21 schwannomas and 2/11 lung tumor metastases; however, oligodendrogliomas and primary malignant lymphomas were not stained. The srp 90 was detected in Western blots of meningioma tissue homogenates. No significant immunohistochemical reaction was seen with sections of normal human cerebra, brain stem, cerebella, pituitary glands and spinal cords. These results document the expression of srp 90 by a variety of primary and metastatic intracranial tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296364
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Stress-response (heat-shock) protein 90 expression in tumors of the central nervous system: an immunohistochemical study (1995)
    Springer
    Acta neuropathologica 89 (1995), S. 184-188 
    Details
    ISSN: 1432-0533
    Keywords: Stress-response protein 90 ; Heat-shock protein 90 ; Brain tumors ; Meningiomas ; Breast tumor metastases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This retrospective study deals with the expression of stress-response (heat-shock) protein 90 (srp 90) in a series of 148 human brain tumors. Immunohistochemical procedures were employed; cells of the human breast cancer line MCF 7 exposed to hyperosmolar stress served as positive controls. Deposits of reaction products were found in the cytoplasm and they displayed a granular pattern. srp 90 was detected in 14/31 meningiomas and 5/10 breast cancer metastases to the brain. The protein was also present in 6/13 glioblastomas and 7/18 astrocytomas. In addition, a positive reaction was found in 2/10 medulloblastomas, 2/14 primitive neuroectodermal tumors, 1/11 pituitary tumor, 2/21 schwannomas and 2/11 lung tumor metastases; however, oligodendrogliomas and primary malignant lymphomas were not stained. The srp 90 was detected in Western blots of meiningioma tissue homogenates. No significant immunohistochemical reaction was seen with sections of normal human cerebra, brain stem, cerebella, pituitary glands and spinal cords. These results document the expression of srp 90 by a variety of primary and metastatic intracranial tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296364
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Alkaline phosphatase isozymes in cultured human cancer cells (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1357-1361 
    Details
    ISSN: 1420-9071
    Keywords: Alkaline phosphatase ; isozymes ; tumor cell lines ; cultured cells ; HeLa cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Alkaline phosphatase, an ubiquitous enzyme is known to exist in several isozymic forms. At least three different isozymes have now been identified in humans. Alkaline phosphatase isozymes are among the substances synthesized ectopically by a variety of human tumors and many continuous cell lines derived from different cancers have retained the capacity to produce these membrane-located glycoproteins. This paper reviews the identification of alkaline phosphatase isozymes in cultured tumor cells and relates these finding with recent developments concerning these cell membrane located glycoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949989
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    Paper (German National Licenses)
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