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  • 1995-1999  (2)
  • 1985-1989
  • Desiccated somatic embryo  (1)
  • Hairy root  (1)
  • 1
    ISSN: 1432-203X
    Keywords: Key words Adventitious bud formation ; Armoracia ; Hairy root ; Bud formation ; Phytochrome A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA of the gene for phytochrome A from Arabidopsis thaliana was fused with the 35S promoter of cauliflower mosaic virus (CaMV35S-PHYA) and introduced into horseradish (Armoracia rusticana Gaert., Mey. et Scherb.) hairy roots. The phytochrome level in hairy roots transformed with CaMV35SPHYA was about three times greater than that in normal hairy roots and the rate of light-induced formation of adventitious shoots was also higher in the hairy roots transformed with CaMV35SPHYA. These results indicate that the light-induced formation of adventitious shoots on horseradish hairy roots is closely related to the phytochrome level.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Key words Carrot ; Abscisic acid ; Freezing preservation ; Desiccated somatic embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have previously reported that strong desiccation tolerance in carrot somatic embryos can be achieved by treatment with abscisic acid (ABA). In this study, we examined the possibility of long-term preservation of ABA-treated and desiccated somatic embryos. Somatic embryos that had been desiccated after treatment with ABA survived for at least 169 weeks at –25 °C. By contrast, somatic embryos that had not been desiccated after treatment with ABA survived for at least 24 weeks at +5 °C but died at –25 °C.
    Type of Medium: Electronic Resource
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