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Specific Degradation of D1 Protein during Exposure of Thylakoid Membranes to High Temperature in the Dark (1999)

Singh, A.K. ; Singhal, G.S.

Springer

Photosynthetica 36 (1999), S. 433-440

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ISSN: 1573-9058

Keywords: antibody ; polyacrylamide gel electrophoresis ; protease ; Triticum aestivum ; wheat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Exposure of thylakoid membranes to high temperature in dark leads to the degradation of D1 protein. Maximum degradation of D1 protein occurred at 45 °C. Using N-terminal specific D1 antibody, a 23 kDa fragment of D1 protein was detected. The degradation of D1 protein could be prevented both by radical scavengers and inhibitors of serine protease and metallo-protease. These results suggest that degradation of D1 protein during exposure of thylakoid membranes to high temperature in dark is catalyzed by protease.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007080204112

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