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  • 1
    Electronic Resource
    Electronic Resource
    Exogenous application of abscisic acid or triadimefon affects the recovery of Zea mays seedlings from heat shock (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 73 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Maize seedlings (Zea mays L. cv. DK 246) grown for 1–4 days in the presence of abscisic acid (ABA) or triadimefon (a fungicide) demonstrated an enhanced ability to withstand the effects of a 3-h sub-lethal (40°C) or lethal (45°C) heat shock. Both the ABA and triadimefon treatments were applied solely to the roots of seedlings; however, the ability to withstand a heat shock was induced in both the root and the shoot. The level of protection provided by these agents was dependent upon the time that plants were exposed to them; prolonged exposure reduced tolerance to subsequent stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb09188.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A Neurospora crassa heat-shocked cell lysate translates homologous and heterologous messenger RNA efficiently, without preference for heat shock messages (1988)
    Springer
    Current genetics 13 (1988), S. 401-409 
    Details
    ISSN: 1432-0983
    Keywords: In vitro translation ; Neurospora ; Heat shock ; Heterologous mRNA translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell-free protein synthesis systems were prepared from normally-grown (N-lysate) and heat-shocked (HS-lysate) Neurospora crassa mycelium. Although both lysates translated homologous mRNA, the HS-lysate was more active, yielding a higher incorporation of [35S]-methionine into hot TCA-insoluble material and a vastly superior protein synthesis profile. The optimal temperature for translation by both lysates was 21 °C; the HS-lysate did not translate heat-shock mRNA preferentially at any temperature tested. Fortuitously, heterologous messenger RNAs from diverse eukaryotic and viral sources — Drosophila, dog pancreas, rabbit globin mRNA, brome mosaic virus, tobacco mosaic virus — were translated by the HS-lysate with an efficiency comparable to that of the commercial rabbit reticulocyte system and superior to the wheat germ system. The cap analogues, m7G(5′)ppp(5′)G and m7G(5′)ppp(5′)Gm, inhibited translation significantly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365661
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence repeat-induced disruption of the major heat-inducible HSP70 gene of Neurospora crassa (1995)
    Springer
    Current genetics 29 (1995), S. 18-26 
    Details
    ISSN: 1432-0983
    Keywords: Heat shock ; Repeat-induced point mutations ; Thermotolerance ; N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The process of repeat-induced point mutation (RIP) was used to disrupt hsps-1, the gene encoding the major heat-inducible member of the HSP70 family of Neurospora crassa. A plasmid DNA, containing an incomplete copy of hsps-1 and the selectable marker qa-2+, was introduced into germinated conidia. The sexual progeny of transformants with ectopically integrated hsps-1 DNA was examined for RIP by Southern-blot analysis of MboI- and Sau3A-digested genomic DNA. Progeny strains, showing RIP, were tested for heat shock-responsive expression of hsps-1, by RNA-blot hybridization and Western-blot analysis, as well as for thermotolerance. Isolates with RIP showed low levels of hsps-1 mRNA and a lack of induction of HSP70 protein by heat shock, accompanied by only a marginal decrease in the acquisition of thermotolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313189
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  • 4
    Electronic Resource
    Electronic Resource
    Organization of chloroplast ribosomal RNA genes and in vitro self-splicing activity of the large subunit rRNA intron from the green alga Chlorella vulgaris C-27 (1997)
    Springer
    Current genetics 31 (1997), S. 503-510 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsChlorella ; rrn23 ; Self-splicing ; Group I-intron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequencing of the rRNA gene (rrn) cluster of Chlorella vulgaris C-27 chloroplasts has revealed a striking organizational difference in comparison to another species of the same genus, Chlorella ellipsoidea C-87. The rrn23 gene in C. vulgaris is also split. However, the 815-bp intervening sequence in this gene has been identified as a group-I intron. An in vitro rrn23 transcript containing the entire intron and parts of flanking exon sequences is able to self-splice in vitro in the presence of GTP and Mg++. Accurate ligation of the exons has been confirmed by sequencing the cDNA of the spliced products. GTP labelling of total Chlorella RNA in vitro has revealed that the number of self-splicing RNAs present in Chlorella chloroplasts is limited compared to that found in other green algal species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050237
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  • 5
    Electronic Resource
    Electronic Resource
    A study of the messenger RNA encoding pyruvate kinase ofNeurospora crassa (1986)
    Springer
    Bioscience reports 6 (1986), S. 201-208 
    Details
    ISSN: 1573-4935
    Keywords: Pyruvate kinase ; messenger RNA ; Northern blots ; in vitro translation ; Neurospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract InNeurospora crassa, there is a single pyruvate kinase (PK) consisting of four identical subunits of ∼60k daltons. Northern and dot blot hybridization studies, using most of the yeast pyruvate kinase gene as a probe, suggest the presence of two distinct mRNA species for pyruvate kinase, separable on the basis of the length of their polyadenylated tails, by oligo(dT)cellulose chromatography. These messages are present in polysomes, immuno-precipitated by anti-PK antibodies, indicating probable translation in vivo. Fractions containing both messages were translatedin vitro in the heterologous systems as well as in a homologousN. crassa lysate, the newly-synthesized PK being detected by immunoadsorption. Protection studies using S1-nuclease suggest no major structural differences in the 5′-untranslated and most of the coding regions of the two messages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01115007
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Relation between processing, microstructure and mechanical properties of rheocast Al-Cu alloys (1988)
    Springer
    Journal of materials science 23 (1988), S. 823-829 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The role of microstructure in modifying the tensile strength of rheocast Al-10 wt % Cu and Al-4.5 wt % Cu is investigated by modelling these products as particulate composites. The tensile strength of rheocast AI-10 wt %Cu alloy is found to depend on experimentally determinable parameter $$\bar D\bar Z$$ which is an undefined function of the size distribution of proeutectic α-particles. These products, however, possess lower strength than the conventional casting at equal porosity level. The tensile strength of the rheocast AI-4.5wt%Cu alloy shows an inverse square root dependence on the proeutectic α-particle size, $$\bar D$$ , and therefore appropriate rheocasting process parameters may be employed to bring about grain refinement in order to produce rheocastings possessing higher strength than the conventional casting at equal porosity level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01153973
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    Paper (German National Licenses)
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