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  • 1995-1999  (1)
  • 1980-1984  (1)
  • Aerobic nitrate respiration  (1)
  • Aspartate aminotrans-ferase  (1)
  • 1
    ISSN: 1432-072X
    Keywords: Keywords Denitrification ; Pseudomonas putida ; Nitrate reductase ; Periplasm ; Aerobic nitrate respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain of Pseudomonas putida that can express a nitrate reductase that is located in the periplasmic compartment was isolated from freshwater. The enzyme was active in vivo during arginine fermentation and at the onset of oxygen limitation in batch cultures. The activity of the enzyme increased the yield of bacteria following fermentative growth under anoxic conditions with arginine, but nitrate reduction did not support growth on non-fermentable carbon substrates under anoxic conditions. Cells expressing the periplasmic nitrate reductase were capable of reducing nitrate in the presence of oxygen. Nitrate reduction under oxic conditions was clearly coupled to a respiratory electron transport chain because: (1) the process was sensitive to the respiratory inhibitors rotenone and 2-n-heptyl-4-hydroxyquinoline N-oxide, and (2) membrane-bound and periplasmic cytochromes were involved. This is the first report of the presence of a periplasmic nitrate reductase in a member of the γ proteobacteria.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 49 (1982), S. 179-186 
    ISSN: 1439-6327
    Keywords: Plasma enzymes ; Creatine kinase ; Aspartate aminotrans-ferase ; Ultra-marathon running
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A number of blood biochemical parameters, including the activities of the plasma enzymes creatine kinase (CK), aspartate amino-transferase (ASAT), lactate dehydrogenase and alkaline phosphatase, were measured in 23 athletes before, and immediately after a 56-km running race. Of the 23 athletes, 18 had previously completed standard 42-km marathon or longer (up to 90-km) ultra-marathon races, whereas not one of the other five athletes had previously run in a long-distance race. After the race, plasma CK and ASAT activities had both risen at least 280% more in the novice runners despite their much slower mean running speed (9.8±0.4 vs. 13.8±0.3 hm/h). There were no other inter-group differences in the absolute levels of the other measured biochemical parameters, although the rise in plasma calcium during the race was significantly greater in the experienced marathon runners. This study shows that either higher levels of training, or previous ultra-marathon racing experience, or both, is associated with lower immediate post-exercise levels of plasma enzyme activity. This is compatible with the finding that physical training reduces post-exercise plasma enzyme levels.
    Type of Medium: Electronic Resource
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