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  • 1995-1999  (1)
  • 1980-1984  (1)
  • Aerobic nitrate respiration  (1)
  • Continuous flow record  (1)
  • 1
    ISSN: 1432-072X
    Keywords: Keywords Denitrification ; Pseudomonas putida ; Nitrate reductase ; Periplasm ; Aerobic nitrate respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain of Pseudomonas putida that can express a nitrate reductase that is located in the periplasmic compartment was isolated from freshwater. The enzyme was active in vivo during arginine fermentation and at the onset of oxygen limitation in batch cultures. The activity of the enzyme increased the yield of bacteria following fermentative growth under anoxic conditions with arginine, but nitrate reduction did not support growth on non-fermentable carbon substrates under anoxic conditions. Cells expressing the periplasmic nitrate reductase were capable of reducing nitrate in the presence of oxygen. Nitrate reduction under oxic conditions was clearly coupled to a respiratory electron transport chain because: (1) the process was sensitive to the respiratory inhibitors rotenone and 2-n-heptyl-4-hydroxyquinoline N-oxide, and (2) membrane-bound and periplasmic cytochromes were involved. This is the first report of the presence of a periplasmic nitrate reductase in a member of the γ proteobacteria.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 19 (1981), S. 373-376 
    ISSN: 1741-0444
    Keywords: Continuous flow record ; Coronary blood flow ; Doppler ultrasonic record of blood flow ; Hindlimbs ; Kidney ; Treadmill exercise
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Continuous recording of blood flow to the coronary, renal and hindlimb vascular beds of sheep has been carried out using flow probes implanted around the major vessels supplying these regions. Blood flow has been recorded at rest, with the sheep running at speeds of 1–16 kmh−1 on a treadmill, and during the recovery period. A Doppler flowmeter has been developed to facilitate compatibility with existing equipment, to enable reliable reproduction of the unit and to keep costs to a minimum. To minimize interference accompanying the violent movements of exercise great care must be taken with the electrical connections and flow probes require streamlining to facilitate implantation. Blood flow to both hindlimb and coronary vascular beds increased during exercise while that to the renal vascular bed did not change. Thus continuous recording with the Doppler ultrasonic flowmeter can be utilised during all phases of exercise, unlike other methods such as the use of radioactive microspheres which provides only point estimations and has limited application for repeated measurement.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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