Electronic Resource
Springer
International journal of legal medicine
112 (1999), S. 134-135
ISSN:
1437-1596
Keywords:
Key words HUMTH01
;
STR polymorphism
;
Allele specific primer
;
APLP method
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Law
Notes:
Abstract We present a simple and rapid technique for determination of alleles at the HUMTH01 locus. The amplified product length polymorphism (APLP) method using two primers different in length, permits the differentiation between allele 9.3 and other alleles. The primers were designed to have an allele-specific nucleotide at the 3′ terminal and 11 non-complementary nucleotides were added to the 5′ terminal of one of the primers for the allele 9.3. The amplified fragment sizes for the alleles 9.3 and 10 were 80 bp and 70 bp, respectively. This method has proved to be very useful for forensic applications.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004140050216
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