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  • 11
    ISSN: 1432-203X
    Keywords: Key wordsM. truncatula ; M. falcata ; MsEnod12A ; MsEnod12B ; Srglb3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R108-1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all explants within 3–4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Boundary layer meteorology 28 (1984), S. 51-77 
    ISSN: 1573-1472
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The ECLATS experiment was conducted in order to investigate the influence of radiative processes on the dynamics of the atmospheric boundary layer during its diurnal evolution. This experiment was carried out over Niger, near Niamey, by measuring continuously the energy balance at ground level and by using an instrumented aircraft for turbulence, radiative fluxes and aerosol measurements in the boundary layer during dusty conditions (‘brumes sèches’). This paper is restricted to an analysis of the turbulent structure in the homogeneous and stationary convective boundary layer. The turbulence moments for kinetic energy and the spectral characteristics of the vertical velocity are discussed. These results are compared with a set of data obtained for clear convective boundary layers. The differences observed are quite important and seem, at least in part, due to radiative processes (infrared radiative divergence in the surface layer and absorption of solar radiation in the boundary layer).
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 1573-0867
    Keywords: soil system ; soil horizon ; building block ; mixing model ; digital elevation model ; TOPMODEL ; hillslope mechanistic model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The hydrology and water quality in landscapes with hydromorphic soils depends on the space and time extension of wetland areas and on water pathways within the landscape at different scales. To study the control of nitrate fluxes by these areas, investigations were carried out on a detailed study site - the Coët Dan catchment (1200 ha) in Brittany, France - involving various disciplines: pedology, soil physics, hydrology, geochemistry and agronomy. An attempt of functional modelling at different hierarchical levels from the horizon level (i - 1) to the region level (i + 3) of soil distribution, extension of saturated areas, horizons physical characteristics, water transfer in a multilayer soil profile and nitrate fluxes was carried out. The soil system, which can be described as a spatial arrangement of a limited number of horizon types with genetic relationships, is tightly controlled by topography. Predictive models of hydromorphic soil distribution using different topographic indexes and DEM were established. Regarding to their hydrodynamic properties, horizons of the soil system have been classified into “building blocks”, which allows to define physically based parameters for a two-dimensional multilayer water transfer model. A four compartment model of flood genesis based on chemical data obtained from different parts of the catena and from the river was coherent with the multilayer hydrodynamic model. The mean nitrate concentrations in several subcatchments were negatively correlated with the percentage of hydromorphic soils. These studies reveal that the hydromorphic zones had an effect on the nitrogen transfer in the catchment, but this effect is limited by the importance of water pathways by-passing the buffer zones. The conclusions of this programme have direct outcomes for designing new landscape management options.
    Type of Medium: Electronic Resource
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