Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 21 (1999), S. 183-192 
    ISSN: 1573-0603
    Keywords: Cell culture ; Clam ; Mollusc ; Oyster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present study attempts to establish cell culture systems for the oyster, Crassostrea gigas Röding and the hard clam, Meretrix lusoria Thunberg. Treatment with collagenase was better than trypsin at dissociating mollusc tissue fragments for in vitro culture. Heart tissue of oyster and hard clam proved to be the most promising target tissue for the establishment of cell lines in vitro. Primary cultures of clam heart were established and successfully maintained for more than 5 months. Collagenase at a concentration of 100 μg/ml may enhance the growth of oyster and hard clam heart cell cultures in vitro.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 21 (1999), S. 199-206 
    ISSN: 1573-0603
    Keywords: In vitro ; Cell culture ; Prawn tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Monolayer cultures were established from ovary, heart, lymphoid tissue and peripheral hemocytes of penaeid shrimps including Penaeus monodon, P. japonicus and P. penicillatus. The most favorable conditions for the culture of penaeid shrimp cells in vitro was in CMRL and L-15 tissue culture media when used within an osmolarity range of 620--760 mmol/kg. The optimal maintenance temperature was 25 °C for tissues of P. japonicus and 28 °C for tissues of P. monodon and P. penicillatus. Among the four tissues tested, lymphoid tissue, or 'Oka organ', was superior to the other tissues for the formation of confluent cell monolayers. Cell cultures from lymphoid tissue and ovary have been subcultured up to three times. When peripheral hemocytes and heart were cultured, a maximum survival of 4 days was obtained. In contrast, cell cultures derived from ovary and lymphoid tissue were maintained alive for at least 20 days in appropriate culture systems. Neither confluent cell sheet nor adherence of cells was obtained in cultivation of hepatopancreas using the present culture systems. The results obtained from the present study also revealed that ovary extract, muscle extract and lobster hemolymph enhanced the survival of the cultured cells of penaeid shrimp in vitro. When the 'Oka organ' cell monolayer was incubated with either white spot disease virus (WSDV) or yellow head virus (YHV), no cytopathic effect (CPE) was obtained. However, at 5--7 days after establishment, significant CPE (a few foci) was observed in cell monolayers derived from WSDV- and YHV-infected Oka tissue. By electron microscopy, virions of WSDV and YHV were observed in the nuclei and cytoplasm of cultured cells. The CPE foci developed further with increased incubation time.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 13 (1997), S. 495-510 
    ISSN: 1069-8299
    Keywords: classical deformation ; shear deformation ; axisymmetric circular plates ; unified finite elements ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper a unified finite element model that contains the Euler-Bernoulli, Timoshenko and simplified Reddy third-order beam theories as special cases is presented. The element has only four degrees of freedom, namely deflection and rotation at each of its two nodes. Depending on the choice of the element type, the general stiffness matrix can be specialized to any of the three theories by merely assigning proper values to parameters introduced in the development. The element does not experience shear locking, and gives exact generalized nodal displacements for Euler-Bernoulli and Timoshenko beam theories when the beam is homogeneous and has constant geometric properties. While the Timoshenko beam theory requires a shear correction factor, the third-order beam theory does not require specification of a shear correction factor. An extension of the work to axisymmetric bending of circular plates is also presented. A stiffness matrix based on the exact analytical form of the solution of the first-order theory of circular plates is derived. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 951-985 
    ISSN: 0029-5981
    Keywords: time-domainBEM ; 2-D convoluted kernel ; transient wave ; quadratic temporal variation ; elastodynamics ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A quadratic time-domain Boundary Element Method (BEM) for two-dimensional (2-D) elastodynamic transient wave analysis is presented. Emphasis is focused on developing time-domain fundamental convoluted kernels and methodology for quadratic temporal solution procedure which are never presented before. In the presented BEM method, the displacement temporal variation is assumed to be quadratic, the traction temporal variation is assumed to be linear (called QL method, a two-time-step piecewise continuity method), and the spatial variations are assumed to be quadratic variation. The QL method is compared with the LC method and the QC method by solving several example problems. Numerical study reveals that the QC and QL methods are more accurate and stable than the LC method, and the QL method is much better than the QC method for transient problems.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 22 (1998), S. 425-447 
    ISSN: 0363-9061
    Keywords: closed-form solution ; transversely isotropic half-space ; Fourier transform ; Hankel transform ; rock anisotropy ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: We rederive and present the complete closed-form solutions of the displacements and stresses subjected to a point load in a transversely isotropic elastic half-space. The half-space is bounded by a horizontal surface, and the plane of transverse isotropy of the medium is parallel to the horizontal surface. The solutions are obtained by superposing the solutions of two infinite spaces, one acting a point load in its interior and the other being free loading. The Fourier and Hankel transforms in a cylindrical co-ordinate system are employed for deriving the analytical solutions. These solutions are identical with the Mindlin and Boussinesq solutions if the half-space is homogeneous, linear elastic, and isotropic. Also, the Lekhnitskii solution for a transversely isotropic half-space subjected to a vertical point load on its horizontal surface is one of these solutions. Furthermore, an illustrative example is given to show the effect of degree of rock anisotropy on the vertical surface displacement and vertical stress that are induced by a single vertical concentrated force acting on the surface. The results indicate that the displacement and stress accounted for rock anisotropy are quite different for the displacement and stress calculated from isotropic solutions. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 22 (1998), S. 495-508 
    ISSN: 0363-9061
    Keywords: consolidation ; cohesive soil ; clay ; axial strain ; true triaxial ; finite difference method ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: This paper presents a simplified approach for the analysis of axial strain induced by three-dimensional consolidation of cohesive soils. The axial strain is divided into a constant volume component and a consolidation component. A relevant undrained stress-strain relationship is required to determine the constant volume component. A theoretical formulation is developed for the evaluation of the consolidation component. Predictions of the axial strain accompanying true triaxial laboratory tests for a variety of stress patterns correlate sufficiently well with the measured data. The proposed method is potentially applicable in conjunction with a finite difference scheme to analyze the time-dependent response of pile groups subjected to static vertical loading. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...