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The effect of methylmercury (CH3HgCl) on the production of endothelium-derived relaxing factor (EDRF) by cultured human umbilical vascular endothelial cells based on its anti-aggregatory effect on human platelets (1995)

Ohno, M. ; Kishimoto, T. ; Tada, M.

Springer

Cell biology and toxicology 11 (1995), S. 303-311

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ISSN: 1573-6822

Keywords: endothelium-derived relaxing factor (EDRF) ; methylmercury ; platelet aggregation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The effect of methylmercury (CH3HgCl) on the production of endothelium-derived relaxing factor (EDRF) by cultured human umbilical vascular endothelial cells (HUVECs) based on its antiaggregatory effect on human platelets was examined. HUVECs were harvested from umbilical veins by collagenase treatment. The platelet aggregation test was performed with cuvettes lined with HUVECs. Platelet aggregation induced by 0.05 units thrombin/ml was inhibited in the presence of HUVECs. This HUVEC-dependent anti-platelet aggregatory effect was enhanced by the addition of bradykinin (10 nmol/L), which stimulates the production of EDRF. Indomethacin (IND, 1 μmol/L) reduced the HUVEC-dependent anti-platelet aggregatory effect. The effect ofN G-monomethyl-L-arginine (L-NMMA, 100 μmol/L), an inhibitor of nitric oxide synthase (NOS) in endothelial cells, on HUVECs pretreated with IND showed almost complete platelet aggregation similar to results without HUVECs. The anti-platelet aggregatory effect of HUVECs pretreated with IND seemed to depend mainly on EDRF. Methylmercury (MeHg) (20–50 μmol/L) induced dose-dependent platelet aggregation in cuvettes, without HUVECs. Methylmercury (30 μmol/L) induced less platelet aggregation in the presence of HUVECs than in their absence. The degree of inhibitory effect by HUVECs on MeHg-induced platelet aggregation was reduced dose-dependently (30–50 μmol/L MeHg). Methylmercury-induced platelet aggregation at 50 μmol/L MeHg with or without HUVECs was similar. These findings suggest that this simple new experimental system is useful for assessing the production of EDRF by HUVECs, and show that MeHg inhibits the production of EDRF by HUVECs, which may be involved in the etiology of cardiovascular diseases such as hypertension and arteriosclerosis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01305903

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Isolation and characterization of human placental chorionic villar extracellular matrix (1979)

Ohno, M. ; Nagle, R. B. ; Meezan, E. ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Supramolecular Structure 12 (1979), S. 457-466

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ISSN: 0091-7419

Keywords: human placental basement membrane ; extracellular matrix ; human chorionic villar basement membrane ; Life Sciences ; Molecular Cell Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: The cell-free extracellular matrix of human placental chorionic villi has been prepared by a procedure employing extraction of the terminal villar fragments with the detergents Triton X-100 and sodium deoxycholate. The isolated human placental extracellular matrix retains an intact, but collapsed, histoarchitecture, as observed by scanning and transmission electron microscopy. It remains intact, in large part because of the presence of continuous sheets of villar basement membranes and associated interstitial collagen fibers and scattered patches of fibrin. The staining characteristics and chemical composition of the isolated human placental extracellular matrix are similar to those reported for basement membranes in several tissues and indicate the presence of collagen-like and glycoprotein components in this preparation. Gel electrophoresis of urea-SDS-mercaptoethanol extracts of the matrix showed that it consists of several polypeptide components of various molecular weights, some of which are associated into high molecular weight complexes by disulfide bonds.

Additional Material: 3 Ill.