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  • 1
    Electronic Resource
    Electronic Resource
    Comparative genome mapping of Sorghum bicolor (L.) Moench using an RFLP map constructed in a population of recombinant inbred lines (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A restriction fragment length polymorphism (RFLP) linkage map of Sorghum bicolor (L.) Moench was constructed in a population of 137 F6-8 recombinant inbred lines using sorghum, maize, oat, barley and rice DNA clones. The map consists of 10 linkage groups (LGs) and 323 markers, 247 of which (76.5%) were ordered at a LOD score ≥ 3.0. The LGs comprise from 61 (LG A) to 13 markers (J), which range in length from 205 (A) to 55 cM (J) and have a combined total length of 1347 cM. Highly significant distorted segregation was detected at all of the 38 loci in a 103-cM segment of LG A, the allelic ratios in the segment ranging from approximately 3:1 (one end) to 19:1 (middle) to 2:1 (other end). Duplicated loci located in different LGs have been mapped with 55 of the 295 DNA probes used in the study (18.6%). The distribution of these loci does not provide support for the hypothesis that Sorghum bicolor (L.) Moench is of tetraploid origin. Comparison of the map with RFLP maps of maize, rice, and oat produced evidence for sorghum-maize LG rearrangements and homoeologies not reported previously, including evidence that: (1) a segment of maize 5L and a segment of 5S may be homoeologous to sorghum LGA; (2) maize LGs 4 and 6 are partly homoeologous to sorghum LGE; (3) the short arm of maize LG 2 is partly homoeologous to sorghum LGF; (4) maize LG 4 may be partly homoeologous to sorghum LG G; (5) maize LG 5 and sorghum LG G contain a larger amount of homoeologous genetic material than previously indicated; and (6) a short segment of maize LG 1 may be homoeologous to a short segment of sorghum LG I.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.118003225.x
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  • 2
    Electronic Resource
    Electronic Resource
    Suppression of basal insulin secretion by adrenalin in normal man and in patients with insulinomas (1977)
    Springer
    Diabetologia 13 (1977), S. 19-23 
    Details
    ISSN: 1432-0428
    Keywords: Basal insulin secretion ; adrenalin ; alpha blockade ; glucose ; insulinoma ; malignant insulinoma ; proinsulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Basal insulin secretion has been thought to be via a different mechanism from stimulated insulin secretion, partly because it is not similarly suppressed by adrenalin. However, adrenalin normally causes hyperglycaemia, but if it is infused while the plasma glucose is kept constant there is marked suppression of insulin secretion. Sympathetic stimulation modulates basal insulin secretion, and alpha adrenergic blockade impaired the suppression of insulin secretion in response to hypoglycaemia. Four of five benign insulinomas had marked suppression of insulin secretion by adrenalin, but one malignant and one benign insulinoma had little suppression. Both had a raised proportion of their basal plasma insulin as proinsulin, and the impaired suppression of secretion by adrenalin probably signified an undifferentiated tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00996322
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  • 3
    Electronic Resource
    Electronic Resource
    Book reviews (1995)
    Springer
    Neuroradiology 37 (1995), S. 250-250 
    Details
    ISSN: 1432-1920
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578268
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  • 4
    Electronic Resource
    Electronic Resource
    Investigating the relaxation rate, following diazo-2 photolysis, of a skinned trabecular preparation from guinea-pig hypertrophied left ventricle (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 771-777 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac hypertrophy Diazo-2 Guinea-pig Relaxation Troponin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Cardiac hypertrophy in the guinea-pig is not accompanied by a large shift in the expression of the predominant isoform of myosin in the left ventricle; however, in this species, thin filament proteins do change. We examined the relaxation, following laser flash photolysis of the photolabile caged Ca2+ chelator diazo-2, of a skinned trabecular preparation from the left ventricle of guinea-pigs that had undergone abdominal aortic banding. Sham-operated animals were used as controls; no guinea-pigs showed any signs of heart failure. We report that mild cardiac hypertrophy does not affect the relaxation rate of Triton-skinned trabeculae from the guinea-pig. However, there was a 35% reduction in the maximum force generated by trabeculae from the left ventricle of the abdominal aortic-banded animals. Additionally, alterations in key troponin subunits occur in the left ventricle of guinea-pigs with mild hypertrophy. We conclude that the thin filament protein changes do not influence trabecular relaxation rates, even though they probably affect maximal force generation. The cellular membrane systems of the intact guinea-pig heart, which were not a factor in this present study, appear to have an important role in the altered cardiac relaxation rates seen in hypertrophy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900131
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  • 5
    Electronic Resource
    Electronic Resource
    Multi-scale Atmospheric Dispersion Modelling by Use of Adaptive Gridding Techniques (1998)
    Springer
    Environmental monitoring and assessment 52 (1998), S. 225-238 
    Details
    ISSN: 1573-2959
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract An accurate prediction of the transport-reaction behaviour of atmospheric chemical species is required to fully understand the impact on the environment of pollution emissions. Elevated levels of secondary pollutants such as ozone in the lower atmosphere can be harmful to the health of both plants and animals, and can cause damage to property present in the urban environment. Detailed models of pollution mechanisms must therefore be developed through comparisons with field measurements to aid the selection of effective abatement policies. Such models must satisfy accuracy requirements both in terms of the number of species represented, and the spatial resolution of species profiles. Computational expense often compels current models to sacrifice detail in one of these areas. This paper attempts to address the latter point by presenting an atmospheric transport-reaction modelling strategy based upon a finite volume discretisation of the atmospheric dispersion equation. The source terms within this equation are provided by an appropriate reduced chemical scheme modelling the major species in the boundary layer. Reaction and transport discretisations are solved efficiently via a splitting technique applied at the level of the non-linear equations. The solution grid is generated using time dependant adaptive techniques, which provide a finer grid around regions of high spatial error in order to adequately resolve species concentration profiles. The techniques discussed are applied in two dimensions employing emissions from both point and area sources. Preliminary results show that the application of adaptive gridding techniques to atmospheric dynamics modelling can provide more accurately resolved species concentration profiles, accompanied by a reduced CPU time invested in solution. Such a model will provide the basis for high resolution studies of the multiple scale interactions between spatially inhomogeneous source patterns in urban and regional environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005895209480
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation of mitochondrial DNA sequences that distinguish male-sterility-inducing cytoplasms in Sorghum bicolor (L.) Moench (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1180-1187 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male-sterility ; Sorghum bicolor ; Mitochondrial DNA clones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have demonstrated that sorghum DNA sequences of mitochondrial origin can be used to distinguish different male-sterility-inducing cytoplasms. Six DNA clones containing single-copy mitochondrial sequences were hybridized on Southern blots to restriction enzyme-digested DNA of 28 sorghum lines representing sources of different cytoplasmic male-sterility (CMS) groups. Four cytoplasmic types were defined on the basis of the pattern of DNA fragments detected. Similar analyses of 50 additional diverse sorghum accessions suggested that three of the four cytoplasmic types may be diagnostic for CMS. Also, three other cytoplasmic types were discovered. These and other mitochondrial DNA clones may be useful molecular tools for “fingerprinting” sterility-inducing cytoplasms in breeding programs, determining cytoplasmic diversity among germ plasm accessions, and identifying new sources of cytoplasm that induce male sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222941
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  • 7
    Electronic Resource
    Electronic Resource
    Glutamate oxaloacetate transaminase isozymes of the Triticinae: dissociation and recombination of subunits (1977)
    Springer
    Theoretical and applied genetics 50 (1977), S. 47-51 
    Details
    ISSN: 1432-2242
    Keywords: Glutamate Oxaloacetate Transaminase ; Isozymes ; Triticum aestivum ; Subunits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple procedure has been developed for the dissociation of active molecules of glutamate oxaloacetate transaminase (GOT: E.C. 2.6.1.1) into protomers and for the reassociation of the subunits into active enzymes. Results of experiments in which the protomers of genetically controlled electrophoretic variants of GOT of Triticum aestivum and of several related species were dissociated and recombined in crude tissue extracts and in partially purified preparations support the hypothesis that the enzyme exists functionally as a dimer in the Triticinae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273797
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  • 8
    Electronic Resource
    Electronic Resource
    RFLP-based assay of Sorghum bicolor (L.) Moench genetic diversity (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 787-796 
    Details
    ISSN: 1432-2242
    Keywords: Sorghum ; RFLP ; Phylogeny Gene flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sixty-two single-copy sorghum DNA clones were used to compare restriction fragment patterns of 53 sorghum accessions from Africa, Asia and the United States. Included were accessions from five morphological races of the cultivated subspecies bicolor, and four races of the wild subspecies verticilliflorum. From two to twelve alleles were detected with each probe. There was greater nuclear diversity in the wild subspecies (255 alleles in ten accessions) than in the domestic accessions (236 alleles in 37 accessions). Overall, 204 of the 340 alleles (60%) that were detected occurred in both subspecies. Phylogenetic analysis using parsimony separated the subspecies into separate clusters, with one group of intermediate accessions. Though exceptions were common, especially for the race bicolor, accessions classified as the same morphological race tended to group together on the basis of RFLP similarities. Selection for traits such as forage quality may have led to accessions genetically more similar to other races being classified as bicolors, which have a loose, small-grained panicle similar to wild races. Population statistics, calculated using four nuclear and four cytoplasmic probes that detect two alleles each, revealed a low but significant amount of heterozygosity, and showed little differentiation in alleles in the wild and cultivated subspecies. Outcrossing with foreign pollen appears to have been more important than migration via seed dispersal as a mechanism for gene flow between the wild and domestic accessions included in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222013
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  • 9
    Electronic Resource
    Electronic Resource
    Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 591-598 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Triticum aestivum ; Triticum peregrinum ; Chromosome addition lines ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analyses of RFLPs, isozymes, morphological markers and chromosome pairing were used to isolate 12 Triticum aestivum cv Chinese Spring (genomes A, B, and D)-T. peregrinum (genomes Sv and Uv) disomic chromosome addition lines. The evidence obtained indicates that each of the 12 lines contains an intact pair of T. peregrinum chromosomes. One monosomic addition line, believed to contain an intact 6Sv chromosome, was also isolated. A CS-7Uv chromosome addition line was not obtained. Syntenic relationships in common with the standard Triticeae arrangement were found for five of the seven Sv genome chromosomes. The exceptions were 4Sv and 7Sv. A reciprocal translocation exists between 4S1 and 7S1 in T. longissimum and evidence was obtained that the same translocation exists in T. peregrinum. In contrast, evidence for syntenic relationships in common with the standard Triticeae arrangements were found for only one Uv chromosome of T. peregrinum.; namely, chromosome 2Uv. All other Uv genome chromosomes are involved in at least one translocation, and the same translocations were found in the U genome of T. umbellulatum. Evidence was also obtained indicating that the centromeric regions of 4U and 4Uv are homoeologous to the centromeric regions of Triticeae homoeologous group-6 chromosomes, that the centromeric regions of 6U and 6Uv are homoeologous to the centromeric regions of group-4 chromosomes, and that 4U and 4Uv are more closely related overall to Triticeae homoeologous group-6 chromosomes than they are to group-4 chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224563
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 591-598 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Wheat ; Triticum aestivum ; Triticum peregrinum ; Chromosome addition lines ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Analyses of RFLPs, isozymes, morphological markers and chromosome pairing were used to isolate 12 Triticum aestivum cv Chinese Spring (genomes A, B, and D)-T. peregrinum (genomes Sv and Uv) disomic chromosome addition lines. The evidence obtained indicates that each of the 12 lines contains an intact pair of T. peregrinum chromosomes. One monosomic addition line, believed to contain an intact 6Sv chromosome, was also isolated. A CS-7Uv chromosome addition line was not obtained. Syntenic relationships in common with the standard Triticeae arrangement were found for five of the seven Sv genome chromosomes. The exceptions were 4Sv and 7Sv. A reciprocal translocation exists between 4Sl and 7Sl in T. longissimum and evidence was obtained that the same translocation exists in T. peregrinum. In contrast, evidence for syntenic relationships in common with the standard Triticeae arrangements were found for only one Uv chromosome of T. peregrinum; namely, chromosome 2Uv. All other Uv genome chromosomes are involved in at least one translocation, and the same translocations were found in the U genome of T. umbellulatum. Evidence was also obtained indicating that the centromeric regions of 4U and 4Uv are homoeologous to the centromeric regions of Triticeae homoeologous group-6 chromosomes, that the centromeric regions of 6U and 6Uv are homoeologous to the centromeric regions of group-4 chromosomes, and that 4U and 4Uv are more closely related overall to Triticeae homoeologous group-6 chromosomes than they are to group-4 chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050168
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