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  • 1
    Electronic Resource
    Electronic Resource
    Effects of Na+, K+ and Mg2+ on45Ca2+ uptake by pancreatic islets (1978)
    Springer
    Pflügers Archiv 378 (1978), S. 93-97 
    Details
    ISSN: 1432-2013
    Keywords: Calcium uptake ; Insulin release ; Islets of Langerhans ; Lanthanum ; Magnesium ; Pancreatic islets ; Potassium ; Sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Microdissected pancreatic islets of noninbredob/ob-mice were used to study ionic effects on the lanthanum-nondisplaceable45Ca2+ uptake by islet cells. Omission of Mg2+ from the incubation medium had no effect, but the45Ca2+ uptake was increased by omission of Na+ and decreased by omission of K+. Excess Mg2+ (1.2–15 mM) inhibited and excess K+ (4.7–25 mM) stimulated the45Ca2+ uptake in a concentration-dependent manner. Stimulation of45Ca2+ uptake in Na+-deficient islets was associated with an enhancement of the basal insulin release. Total abolishment of glucose-stimulated45Ca2+ uptake in K+-deficient islets did not preclude a significant secretory response to glucose. It is concluded that the lanthanum-nondisplaceable45Ca2+ uptake shows a partial correlation to insulin release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584440
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The dynamics of insulin release from mouse pancreatic islet cells in suspension (1976)
    Springer
    Pflügers Archiv 366 (1976), S. 185-188 
    Details
    ISSN: 1432-2013
    Keywords: Insulin release ; Pancreatic β-cells ; β-Cells in suspension ; Intact pancreatic islets ; Glucose oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1–2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3–20 mM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585876
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    45Ca2+ uptake by dispersed pancreatic islet cells: Effects ofd-glucose and the calcium probe, chlorotetracycline (1979)
    Springer
    Pflügers Archiv 381 (1979), S. 281-285 
    Details
    ISSN: 1432-2013
    Keywords: Calcium uptake ; Chlorotetracycline ; Dispersed cells ; Islets of Langerhans ; Pancreatic β-cells ; Pancreatic Islets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Uptake of45Ca2+ was studied in dispersed pancreatic islet cells from non-inbredob/ob-mice. Like whole islets the dispersed cells responded to 20 mMd-glucose with a markedly increased45Ca2+-labeling of both the lanthanum-nondisplaceable and the lanthanum-displaceable calcium pools. The pronounced effect ofd-glucose could not be reproduced with 3-O-methyl-d-glucose,l-glucose,d-mannose,l-leucine, ord-leucine; however,45Ca2+ uptake was greater in the presence ofl-leucine as compared withd-leucine.45Ca2+ uptake by dispersed cells or whole islets was stimulated severalfold by 100 μM or more chlorotetracycline. At the concentration of only 10 μM, chlorotetracycline had no effect on whole islets and partially inhibited45Ca2+ uptake by the dispersed cells. The ability ofd-glucose to stimulate45Ca2+ uptake by islets or dispersed cells remained in the presence of 10 μM chlorotetracyline. Islet cell suspensions apparently represent a valid model for studying how Ca2+ interacts with the cells. However, when using chlorotetracycline as fluorescent Ca2+ probe, attention must be paid to its potential ionophoric activity. At only 10 μM, the drug seems to monitor a peripheral pool of Ca2+, some of which may reside in normal transport channels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583260
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Initial increase and subsequent loss of vasoactive intestinal polypeptide immunoreactivity in acetylcholinesterase-positive neurons of mouse islets transplanted to the kidney (1996)
    Springer
    Cell & tissue research 284 (1996), S. 391-400 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Acetylcholinesterase ; Islets of Langerhans ; Neuro-insular complex ; Vasoactive intestinal polypeptide (VIP) ; Transplanted pancreatic islets ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Collagenase-isolated pancreatic islets from C57BL/6J mice were cultured overnight and transplanted under the kidney capsule of non-diabetic syngeneic hosts. Cryostat sections of grafts and fresh islets were stained for acetylcholinesterase (AChE) and vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI). Immediately after isolation, as well as 2–5 days after transplantation, VIP-LI- and AChE-positive nerve cell bodies were clearly seen in the periphery of the islets. Grafts 3–5 days old exhibited a transient and marked increase in VIP-LI nerve cell bodies and fibers. Seven days after transplantation VIP-LI nerve structures began to decrease in number and after 26–52 weeks they were no longer detectable. In contrast, AChE-positive nerve cell bodies and fibers, which showed a relatively constant pattern of distribution, were observed throughout the entire observation period. Restaining experiments demonstrated the coexistence of VIP-LI and AChE activity in the neurons. It is concluded that the grafts were extensively equipped with an intrinsic VIP-ergic and AChE-positive innervation. The initial, transient enhancement of VIP-LI expression probably reflects an adaptation of the neuro-insular complex to the preganglionic denervation, or to the ectopic environment, or both.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050600
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    Paper (German National Licenses)
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