ISSN:
1615-6102
Keywords:
Chicory
;
Cichorium
;
Plane of division
;
Somatic embryogenesis
;
Nucleus staining
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary First divisions of embryogenic cells were studied in leaves of plantlets of aCichorium hybrid (C. intybus L. ×C. endivia L.) cultured in vitro in a liquid agitated medium, at 35 °C in the dark. Stages of reactivation of competent cells were characterized by increase of nucleus and nucleolus diameter, migration of the nucleus in the centre of the cell and thickening of the cell wall. The first division of reactivated embryogenic cells was symmetrical and anticlinal in regard to the xylem vessels orientation. Embryogenic structures consisted in I-type tetrads or in rows of 4–8 cells. Then the divisions occurred in thickness at one end, without polarization or formation of a suspensor-like structure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01281326
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