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  • 1995-1999  (2)
  • 1970-1974
  • Cell wall  (1)
  • Embryo sac  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Rethinking cytoskeleton in plant reproduction: toward a biotechnological future? (1999)
    Springer
    Sexual plant reproduction 12 (1999), S. 67-70 
    Details
    ISSN: 1432-2145
    Keywords: Key words Cytoskeleton ; Cytoskeleton proteins ; Cytoskeleton function ; Pollen tube ; Embryo sac
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sexual reproduction in plants is intimately connected to the activity of the cytoskeletal apparatus in reproductive cells. Because of the ease with which the pollen tube can be studied, it has become a model for studying many aspects of cell physiology related to the cytoskeleton, such as movement of organelles and vesicles and cell division. However, information about cytoskeletal proteins is still insufficient for determining cytoskeletal functions during reproduction, especially in terms of cell-cell interactions. One reason may be that cytological and biochemical research on the cytoskeleton of pollen and the embryo sac has not been complemented by sufficient research activity at genetic and molecular levels, and few laboratories are currently involved in this work. This might be because of problems in identifying appropriate applied applications of the work that might attract more investigation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050173
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural immunolocalization of periodic pectin depositions in the cell wall ofNicotiana tabacum pollen tubes (1995)
    Springer
    Protoplasma 187 (1995), S. 168-171 
    Details
    ISSN: 1615-6102
    Keywords: Cell wall ; Immunocytochemical localization ; JIM5 antibody ; Pectins ; Pollen tube
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The monoclonal antibody (MAb) JIM5, marking acidic pectins, was used to localize ultrastructurally pectin molecules in the pollen tube wall ofNicotiana tabacum. Longitudinal sections of LR-White embedded pollen tubes were exposed to antibody treatment; accumulations of pectins were identified by counting the density of the gold particles representing the pectin epitopes along the pollen tube wall. Significant accumulations of gold grains were marked and the distances between them were measured. In many pollen tubes a more or less regular distribution of the accumulations was observed along the tube indicating a periodical deposition of pectin. The distances between the accumulations were 4–6 μm. Most of the label was found in the inner part of the outer layer of the bilayered cell wall. These findings correspond to and confirm the earlier observation by our group reporting ring-shaped periodical deposits in pollen tubes after immunofluorescence labelling with the MAb JIM5 under the confocal laser scanning microscope.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280245
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    Paper (German National Licenses)
    Fulltext
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