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  • 1
    Electronic Resource
    Electronic Resource
    Effects of temperature on human L-type cardiac Ca2+ channels expressed in Xenopus oocytes (1998)
    Springer
    Pflügers Archiv 436 (1998), S. 238-247 
    Details
    ISSN: 1432-2013
    Keywords: Key words Calcium ; Heart ; Hypothermia ; Phosphorylation ; Protein kinase A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Temperature normally affects peak L-type Ca2+ channel (CaCh) current with a temperature coefficient (Q 10) of between 1.8 and 3.5; in cardiomyocytes attenuating protein kinase A activity increases Q 10 whilst activating it lowers Q 10. We examine temperature effects using cloned human cardiac CaChs expressed in Xenopus oocytes. Peak inward currents (I Ba) through expressed CaChs (i.e. α1Cα2/δaβ1b) exhibited a Q 10 of 5.8±0.4 when examined between 15 and 25°C. The nifedipine-sensitive I Ba exhibited a higher Q 10 of 8.7±0.5, whilst the nifedipine-insensitive I Ba exhibited Q 10 of 3.7±0.3. Current/voltage (I/V) relationships shifted to negative potentials on warming. Using instead a different CaCh β subunit isoform, β2c, gave rise to an I Ba similar to those expressed using β1b. We utilized a carboxyl deletion mutant, α1C-Δ1633, to determine the temperature sensitivity of the pore moiety in the absence of auxiliary subunits; I Ba through this channel exhibited a Q 10 of 9.3±0.3. However, the Q 10 for macroscopic conductance was reduced compared to that of heteromeric channels; decreasing from 5.0 (i.e. α1Cα2/δaβ1b) and 3.9 (i.e. α1Cα2/δaβ2c) to 2.4 (α1C-Δ1633). These observations differ markedly from those made in studies of cardiomyocytes, and suggest that enhanced sensitivity may depend on the membrane environment, channel assembly or other regulatory factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050628
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    GENERAL BOUNDARY ELEMENT METHOD FOR NON-LINEAR PROBLEMS (1996)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 23 (1996), S. 467-483 
    Details
    ISSN: 0271-2091
    Keywords: general BEM ; non-linear operators ; homotopy ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this paper the well-known non-linear equation f‴+½ff″=0 with boundary conditions f(0)=0, f′(0)=0 and f (∞)=1 is used as an example to describe the basic ideas of a kind of general boundary element method for non-linear problems whose governing equations and boundary conditions may not contain any linear terms at all.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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