Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1995-1999  (4)
  • 1965-1969  (3)
  • 1950-1954
  • 1930-1934  (1)
  • Chemistry  (4)
  • Inorganic Chemistry  (4)
  • Amiloride  (2)
  • Cell & Developmental Biology  (2)
  • Life and Medical Sciences  (2)
Source
Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    cAMP-activation of amiloride-sensitive Na+ channels from guinea-pig colon expressed in Xenopus oocytes (1996)
    Springer
    Pflügers Archiv 431 (1996), S. 913-922 
    Details
    ISSN: 1432-2013
    Keywords: Key wordsXenopus oocytes ; Guinea-pig colon ; Na+ channel ; Single-channel analysis ; Amiloride ; Benzamil ; cAMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Guinea-pig distal colonic mRNA injection into Xenopus laevis oocytes resulted in expression of functional active epithelial Na+ channels in the oocyte plasma membrane. Poly(A)+ RNA was extracted from distal colonic mucosa of animals fed either a high-salt (HS) or a low-salt (LS) diet. The electrophysiological properties of the expressed amiloride-sensitive Na+ conductances were investigated by conventional two-electrode voltage-clamp and patch-clamp measurements. Injection of poly(A)+ RNA from HS-fed animals [from hereon referred to as HS-poly(A)+ RNA] into oocytes induced the expression of amiloride-sensitive Na+ conductances. On the other hand, oocytes injected with poly(A)+ RNA from LS-fed animals [LS-poly(A)+ RNA] expressed a markedly larger amount of amiloride-blockable Na+ conductances. LS-poly(A)+ RNA-induced conductances were completely inhibitable by amiloride with a K i of 77 nM, and were also blocked by benzamil with a K i of 1.8 nM. 5-(N-Ethyl-N-isopropyl)-amiloride (EIPA), even in high doses (25 μM), had no detectable effect on the Na+ conductances. Expressed amiloride-sensitive Na+ channels could be further activated by cAMP leading to nearly doubled clamp currents. When Na+ was replaced by K+, amiloride (1 μM) showed no effect on the clamp current. Single-channel analysis revealed slow gating behaviour, open probabilities (P o) between 0.4 and 0.9, and slope conductances of 3.8 pS for Na+ and 5.6 pS for Li+. The expressed channels showed to be highly selective for Na+ over K+ with a permeability ratio P Na/P K 〉 20. Amiloride (500 nM) reduced channel P o to values 〈 0.05. All these features make the guinea-pig distal colon of LS-fed animals an interesting mRNA source for the expression of highly amiloride-sensitive Na+ channels in Xenopus oocytes, which could provide new insights in the regulatory mechanism of these channels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050085
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Cystic fibrosis and non-cystic-fibrosis human nasal epithelium show analogous Na+ absorption and reversible block by phenamil (1997)
    Springer
    Pflügers Archiv 434 (1997), S. 19-24 
    Details
    ISSN: 1432-2013
    Keywords: Key words Cystic fibrosis ; Human nasal epithelium ; Primary culture ; Epithelial Na+ channel ; Amiloride ; Phenamil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Transepithelial short-circuit current (I SC), potential (V T) and resistance (R T) of confluent monolayers of human nasal epithelium cultured from patients with and without cystic fibrosis (CF) were measured. In our Ussing chamber experiments with monolayers derived from non-CF and CF patients neither I SC (non-CF: 14.1 ± 1.0 μA/cm2, n = 77; CF: 16.7 ± 1.5 μA/cm2, n = 42), nor R T (non-CF: 288 ± 15 Ω· cm2; CF: 325 ± 20 Ω· cm2) showed any significant differences, only V T showed moderate but significant different values (non-CF: –3.6 ± 0.4 mV; CF: –5.6 ± 0.7 mV, respectively). Total I SC in CF cells was nearly completely inhibited by amiloride (92 ± 9.6%), while in non-CF tissue amiloride-insensitive conductances mediated a considerable amount of the I SC (36.3 ± 6.1%), indicating a lower activity of amiloride-sensitive Na+ conductances in non-CF cells. In both tissues the amiloride-sensitive I SC could also be blocked by the amiloride analogues benzamil, phenamil and 5-(N-ethyl-N-isopropyl)2’,4’-amiloride (EIPA) with different affinities. However, amiloride had a significant lower affinity in CF tissue (half-maximal blocker concentration, K 1/2 = 586 ± 59 nM) compared with non-CF tissue (K 1/2 = 294 ± 22 nM). Astonishingly, phenamil, a blocker which irreversibly blocks all epithelial Na+ channels hitherto described, inhibited the Na+ conductances of human nasal epithelium in a completely reversible way, but nevertheless with high affinity (non-CF: K 1/2 = 12.5 ± 1.2 nM; CF: K 1/2 = 17.1 ± 1.1 nM). Even in high doses none of these blockers had any effect on intracellular Ca2+ concentration as measured with Fura-2. From these findings, we conclude that the epithelial Na+ conductances of human CF nasal epithelium show modified regulation or are functionally different from those of other tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050358
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Das Zustandsdiagramm der Kupfer-Goldlegierungen (1931)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 201 (1931), S. 41-74 
    Details
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19312010107
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The in vitro response of thymic lymphocytes of the pig to phytohemagglutininThis work was aided by Pennsylvania Plan Funds, grant FR-00120-01 from the Division of Research Facilities and Resources, and by U.S.P.H.S. grant 5T1 Ca-5097.The work was performed at the interdisciplinary cancer research facilities of the school of veterinary medicine. (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 285-299 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The response of thymic lymphocytes of the pig to phytohemagglutinin was studied with H3 thymidine in cultures, from 0-72 hours. At the beginning of the culture period 6-18% of lymphocytes were in DNA synthesis. during the first 24 hours a sharp decrease in the number of DNA synthesizing cells was observed in both pha and control cultures, although pha cultures consistently showed small but significantly greater numbers of DNA synthesizing cells. this was followed by a definite peak in DNA synthesis and mitotic response of a minority of the cells in pha cultures between 48-54 hours, whereas in control cultures activity ceased. in addition, a small proportion of the progeny of initially DNA synthesizing medium sized lymphocytes was apparently stimulated by pha and found in mitosis by 48 hours.It was concluded that the thymus contains a fraction of lymphocytes, not in the mitotic cycle, which are capable of being transformed by pha to mitotic activity. the data also suggests some stimulation of cells already in the mitotic cycle.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040670209
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Difference between medullary and cortical thymic lymphocytes of the pig in their response to phytohemagglutininThis work was aided by Pennsylvania Plan Funds and U.S.P.H.S. grants FR-00120-02 and 5 TI Ca 5097.Most of this work was performed at the Interdisciplinary Cancer Research Facilities of the School of Veterinary Medicine. (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 68 (1966), S. 117-125 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Pig thymus tissue was cultured with organ culture techniques in the presence and absence of phytohemagglutinin in the culture medium. Morphologic and autoradiographic observations indicate that in the pig, lymphocytes capable of responding to phytohemagglutinin are located predominantly in the medulla of the thymus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040680206
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Die Ammonolyse von (NH4)2GeF6. Darstellung und Struktur von NH4[Ge(NH3)F5] (1997)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 623 (1997), S. 1529-1534 
    Details
    ISSN: 0044-2313
    Keywords: Formation of NH4[Ge(NH3)F5] ; structure ; ammonolysis ; ir spectrum ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Ammonolysis Reaction of (NH4)2GeF6. Synthesis and Structure of NH4[Ge(NH3)F5](NH4)2GeF6 reacts with ammonia to yield NH4[Ge(NH3)F5] at 280°C. The reaction path was elucidated by in situ time and temperature resolved X-ray powder diffraction. NH4[Ge(NH3)F5] crystallizes isostructurally to NH4[Si(NH3)F5] in the tetragonal space group P4/n (No. 85) with lattice constants a = 619.41(1) pm and c = 724.70(1) pm. The germanium atom is coordinated by five fluorine atoms and the nitrogen atom of the ammonia molecule. The ammonium cation is located on the Wyckoff position (2 a) in P4/n. The crystal structure is stabilized by extensive hydrogen bonding.
    Notes: (NH4)2GeF6 reagiert mit Ammoniak bei 280°C zu NH4[Ge(NH3)F5]. Der Reaktionsverlauf wurde in situ mit Hilfe der zeit- und temperaturaufgelösten Röntgenpulverdiffraktometrie verfolgt. Das so dargestellte NH4[Ge(NH3)F5] kristallisiert isostrukturell mit NH4[Si(NH3)F5] in der tetragonalen Raumgruppe P4/n (Nr. 85) mit den Gitterkonstanten a = 619,41(1) pm und c = 724,70(1) pm. Dabei ist das Germaniumatom verzerrt oktaedrisch von fünf Fluoratomen und dem Stickstoffatom des Ammoniakmoleküls koordiniert. Das Ammoniumion liegt auf der Tetraederpunktlage (2 a) in P4/n. Die Verbindung wird durch Wasserstoffbrückenbindungen stabilisiert.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19976231010
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Zur Kenntnis des Phosphorpentachlorids. Über die Reaktion von PCI5 mit aliphatischen Nitrilen (1969)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 367 (1969), S. 40-43 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: The course of interactions betweens some aliphatic nitriles and PCI5 has been investigated by means of 31P—NMR spectroscopy. Phosphazo compounds are formed, and chlorination occurs. Three examples of cis-trans isomeric phosphazo compounds were found.
    Notes: Der Ablauf von Reaktionen einiger aliphatischer Nitrile mit PCI5 wurde mit Hilfe der 31P—KMR-Spektroskopie untersucht. In allen Fällen entstehen Phosphazoverbindungen; außerdem tritt Chlorierung ein. Drei Beispiele von cis-trans-isomeren Phosphazoverbindungen wurden gefunden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19693670106
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Zeit- und temperaturaufgelöste Röntgenpulverdiffraktometrie: Die Reaktion von (NH4)2SnF6 mit Ammoniak (1995)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 621 (1995), S. 617-623 
    Details
    ISSN: 0044-2313
    Keywords: Formation of Sn(NH3)2F4 ; ammonolysis ; thermal decomposition of (NH4)2SnF6 ; time and temperature resolved x-ray diffractometry ; in situ x-ray diffractometry ; solid state reactions ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Time and Temperature Resolved in situ X-Ray Powder Diffractometry. The Reaction of (NH4)2SnF6 with AmmoniaThe thermal decomposition of (NH4)2SnF6 under an atmosphere of ammonia is reported. The complicated reaction paths were illucidated by time and temperature resolved in situ x-ray powder diffractometry. It is shown that this technique is a powerful tool to observe structural changes during reaction. It offers also a valuable access to thermodynamic and kinetic data for solid state and gas phase reactions. (NH4)2SnF6 decomposes under ammonia below room temperature to NH4F and amorphous SnF4 · x NH3. At a temperature of 80°C an intermediate product, (NH4)4SnF8, is formed, which decomposes at 140°C into (NH4)2SnF6 and NH4F. At 250°C (NH4)[Sn(NH3)F5] and Sn(NH3)2F4 are formed. The latter crystallises C-centered monoclinic with lattice constants a = 844.1(5) pm, b = 630.5(3) pm, c = 520.2(3) pm and b̃ = 114.02(7)°. At 330°C a further decomposition yields SnF2(NH2)2 with a C-centered monoclinic cell and lattice constants a = 1 069(7), b = 325.3(2), c = 504.8(3) pm and b̃ = 105.83(7)°. Finally above 500°C tin metal is formed.
    Notes: Der komplizierte Verlauf der Reaktion von NH3 mit (NH4)2SnF6 wird mit der zeit- und temperaturaufgelösten Röntgendiffraktometrie verfolgt. Dabei erweist sich diese Methode als ideale Technik, um Veränderungen in der Struktur während der Reaktion aufzuzeichnen und ebenfalls thermodynamische und kinetische Daten zu gewinnen. (NH4)2SnF6 zersetzt sich schon bei 25°C unter trockenem NH3-Gas zu NH4F und einem röntgenamorphen Ammoniakat SnF4 · x NH3. Ab 80°C bildet sich (NH4)4SnF8 als Zwischenprodukt, das sich ab 140°C wieder in (NH4)2SnF6 und NH4F zersetzt. Bei 250°C entstehen NH4[Sn(NH3)F5] und Sn(NH3)2F4. Letzteres kristallisiert monoklin C-zentriert mit den Gitterkonstanten a = 844,1(5) pm, b = 630,5(3) pm, c = 520,2(3) pm und b̃ = 114,02(7)°. Ab 330°C erhält man SnF2(NH2)2. Aus dem Pulverdiffraktogramm ergibt sich eine C-zentrierte monokline Elementarzelle mit a = 1 069(7), b = 325,3(2), c = 504,8(3) pm und b̃ = 105,83(7)°. Bei noch höheren Temperaturen erfolgt die Zersetzung zu Zinn.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19956210419
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...