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  • 1995-1999  (2)
  • 1965-1969
  • 1830-1839
  • Acari  (1)
  • Bamboo mosaic potexvirus  (1)
  • 1
    ISSN: 1572-994X
    Keywords: Bamboo mosaic potexvirus ; defective RNA ; RNA combination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5′ terminus and 148 nts of the 3′ terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5′ and 3′ junction borders of reported potexvirus D RNAs reveal a 65.2–84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental and applied acarology 21 (1997), S. 273-277 
    ISSN: 1572-9702
    Keywords: Acari ; Phytoseiidae ; sampling ; leaf-washing apparatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Boller's funnel and sieve apparatus for extracting phytoseiid mites (Acari: Phytoseiidae) from leaves was modified. The modification consisted of including a reservoir dish enabling the placing of the whole sample of leaves into the apparatus, attaching a collecting chamber, simplification of the fixing of the fine lower sieve and adding an upper fine sieve. This modification enables the rapid processing of samples of up to 150 leaves.
    Type of Medium: Electronic Resource
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