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  • 1995-1999  (1)
  • 1960-1964
  • 1955-1959
  • 1925-1929
  • Abbreviations: CTAB, cetyltrimethylammonium bromide; DEPC, diethylpyrocarbonate; PVP, polyvinylpyrrolidone; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis  (1)
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  • 1995-1999  (1)
  • 1960-1964
  • 1955-1959
  • 1925-1929
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 15 (1996), S. 516-521 
    ISSN: 1432-203X
    Keywords: Key words: conifer, Picea glauca, RNA extraction ; Abbreviations: CTAB, cetyltrimethylammonium bromide; DEPC, diethylpyrocarbonate; PVP, polyvinylpyrrolidone; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.
    Type of Medium: Electronic Resource
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