ISSN:
1432-1750
Keywords:
Immunohistochemistry
;
l-arginine
;
Nitric oxide
;
Nonadrenergic noncholinergic nerve
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Bradykinin (10−8-10−5M) caused a concentration-dependent increase in cyclic GMP (cGMP) production in bovine tracheal smooth muscle in the absence of epithelium. The effect was calcium-dependent and was inhibited by pyrogallol (10 μM) and methylene blue (10 μM). The inhibition of pyrogallol was reversed by superoxide dismutase (100 Usnowml). Nitric oxide (NO) synthase inhibitors, N G-methyl-l-arginine (10–100 μM) and N G-nitro-l-arginine (10–100 μM) reduced cGMP accumulation induced by bradykinin in a concentration-dependent fashion, and the inhibition was reversed by l-arginine. Immunohistochemistry with a specific antibody against neuronal NO synthase from rat cerebellum showed positive staining localized in some nerve fibers. Bradykinin-induced cGMP accumulation appears to be related to the release of NO, part of which is probably synthesized in nonadrenergic noncholinergic nerve in bovine trachea.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00172144
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