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  • 1995-1999  (2)
  • 1955-1959
  • Key words Nod factor binding  (1)
  • Tetracycline in serum  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A Nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 261-267 
    Details
    ISSN: 1617-4623
    Keywords: Key words Nod factor binding ; Lectin ; Apyrase ; Legume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recent studies from our laboratory have found that a root lectin from the legume Dolichos biflorus is present on the root surface, binds rhizobial Nod factor and has apyrase activity. To assess the broader significance of this lectin/nucleotide phosphohydrolase (Db-LNP), we have cloned a second related cDNA (Db-apyrase-2) from D. biflorus, as well as related cDNAs from the legumes Lotus japonicus and Medicago sativa, and from Arabidopsis thaliana, a non-legume. The deduced amino acid sequences of these apyrases were aligned with one another and with the sequences of other apyrases from plants, animals, yeast and protozoa. Phylogenetic analysis shows that Db-LNP has closely related orthologs only in other legumes, while Db-apyrase-2 is more closely related to apyrase sequences from non-leguminous plants. We also show that the orthologs of Db-LNP from M. sativa and Pisum sativum have carbohydrate binding activity. The results suggest that legume LNPs may represent a special class of apyrases that arose by gene duplication and subsequent specialization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051082
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Simple and rugged SPE method for the determination of tetracycline antibiotics in serum by HPLC using a volatile mobile phase (1997)
    Springer
    Chromatographia 44 (1997), S. 187-190 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Solid phase extraction ; Tetracycline in serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and rugged SPE method for the determination of tetracycline (TC), minocycline (MC) and demeclocycline (DCC) in porcine serum by high performance liquid chromatography (HPLC) was developed. The spiked serum sample was pretreated with 2% phosphoric acid followed by a simple and rugged solid-phase extraction procedure using the OasisTM HLB extraction cartridges. High and reproducible recoveries were obtained even though the cartridges were run dry. The extracted sample analytes were injected onto a Waters SymmetryShieldTM RP8 column. The mobile phase was a simple volatile solution containing 0.1% TFA, 2% methanol and 7% acetonitrile in Water. The antibiotics were detected at 350 nm. The calibration curves were linear from 2.0 to 25.0 μg mL−1 of TC and MC with DCC as the internal standard at a concentration of 25.0 μg mL−1. For six replicate analyses, the average recoveries of TC and MC from porcine serum sample fortified at the level of 2.5 μg mL−1 were 96.1% with 1.3% RSD and 101% with 0.54% RSD; at level of 0.5 μg mL−1 the average recoveries were 88% with 1.6% RSD and 97.8% with 1.4% RSD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02466454
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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