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  • 1995-1999  (2)
  • 1940-1944
  • Conformational analysis  (1)
  • Flow cytometry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    3-Vinyl-1H-indoles: A conformational study, electronic aspects and reactivity predictions forDiels-Alder reactions (1995)
    Springer
    Monatshefte für Chemie 126 (1995), S. 303-317 
    Details
    ISSN: 1434-4475
    Keywords: 3-Vinyl-1H-indoles ; Conformational analysis ; Diels-Alder reactivity ; Quantum chemistry calculations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Konformationsanalysen von einigen 3-Vinyl-1H-indolen, ausgehend von Röntgenstrukturgeometrien, wurden mit den Methoden der Quantenchemie und Kraftfeldmechanik durchgeführt. Die aus quantenchemischen Rechnungen erhaltenen Ladungen, Grenzorbitale und molekularen elektrostatischen Potentiale stellen verläßliche Reaktivitätsindices zur Vorhersage der Ergebnissse vonDiels-Alder-Reaktionen mit 3-Vinyl-1H-indolen dar.
    Notes: Summary On the basis of X-ray geometries, a theoretical conformational study of some 3-vinyl-1H-indoles was performed with quantum chemistry and molecular mechanics programs. Charge, frontier orbitals, and the molecular electrostatic potential derived from quantum chemistry calculations of 3-vinyl-1H-indoles are reliable reactivity parameters to predict the outcome of theDiels-Alder reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00816100
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    HLA-B27 determination using serological methods. A comparison of enzyme immunoassay and a microlymphocytotoxic test with flow cytometry and a molecular biological assay (1996)
    Springer
    Rheumatology international 16 (1996), S. 95-100 
    Details
    ISSN: 1437-160X
    Keywords: HLA-B27 ; Microlymphocytotoxicity test ; Enzyme immunoassay ; Flow cytometry ; Rheumatology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Typing for HLA-B27 is routinely performed in patients with seronegative spondarthritides. Besides the microlymphocytotoxic test (MLCT), other serological techniques have been developed such as enzyme immunoassays (EIA) using serum or plasma as a source for the determination of soluble HLA-B27 (sHLA-B27) and flow cytometric (FC) methods. The aim of the present study was to check the accuracy and reliability of the EIA for sHLA-B27 in comparison to the MLCT using antibodies against HLA-B27 and cross-reacting specificities (CRS), as well as an FC method and a molecular biological method. Any discrepant results should be typed with the MLCT using a complete panel of anti-HLA-class I antibodies, with FC and with a molecular biological technique. The EIA should also be repeated in those patients, using serum and plasma from a new venipuncture. In 81 patients with rheumatic disorders, the EIA and the MLCT using antibodies against HLA-B27 and CRS were performed. Based on the MLCT with a complete panel of anti-HLA-class I antibodies as a standard, discrepant test results were obtained for 9 out of 81 patients with the MLCT using antibodies against HLA-B27 and CRS and with the EIA. The following wrong results occurred: in the MLCT with anti-HLA-B27 and CRS, there were two false-negative results; in the EIA there were four false-negative and one false-positive results: one sample was undeterminable. In comparison with the MLCT, including the complete panel of HLA-class I antibodies, as well as with a molecular biological technique, typing with FC showed a complete concordance. Our investigations demonstrated that for routine typing for HLA-B27 the MLCT cannot be replaced by EIA because of a significant number of mistypings. The MLCT performed only with antibodies against HLA-B27 and CRS may also lead to typing errors. No errors were detected using flow cytometry. If only serological methods can be performed in a laboratory a combination of flow cytometry and MLCT could therefore enhance the safety of HLA-B27 typing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01409980
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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