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Survival strategy of Pistia stratiotes L. in the Djoudj National Park in Senegal (1999)

den Hollander, Nick G. ; Schenk, Ingrid W. ; Diouf, Sara ; [et al.]

Springer

Hydrobiologia 415 (1999), S. 21-27

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ISSN: 1573-5117

Keywords: Pistia stratiotes ; seed formation ; flowering ; habitat factors

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The water level in the Senegal River is now regulated due to the construction of two high dams (1986 and 1988). In consequence, large parts of the floodplains have permanently dried up and the brackish water in the lower part of the river has become fresh. The Djoudj National Park, situated approximately 60 km upstream from Saint Louis in Senegal in the former floodplain, is artificially flooded when inlets from the river are opened during the rainy season. This change in ecological conditions has led to an annually recurrent, explosive development of the floating aquatic weed Pistia stratiotes L. in the Park during the first half of the dry season. A rapid increase in the salt content of the water in the Park, in the course of the dry season (due to the high salt content of the soils and evaporation), eventually causes massive die back of Pistia. Rapid seed production by Pistia permits it to survive these unfavourable conditions, i.e. high salinity coupled with drought during the second half of the dry season. This applies in particular to an area where the water is fertilised by excreta from a pelican colony. Flowering of Pistiaappears to be markedly enhanced when vegetative reproduction is inhibited by crowding.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1003817213753

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Nonradioactive Northern blotting with biotinylated and digoxigenin-labeled RNA probes (1998)

Meltzer, Jon C. ; Sanders, Veronica ; Grimm, Paul C. ; [et al.]

Weinheim : Wiley-Blackwell

Electrophoresis 19 (1998), S. 1351-1355

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ISSN: 0173-0835

Keywords: Stripping ; Chemiluminescence ; Alkaline phosphatase ; Nonradioactive blotting ; Peroxidase ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: The application of nonradioactive RNA probes for Northern blotting offers the advantage of a rapid turn-around time for results without the loss of sensitivity for target mRNA detection. However, a problem that has impeded the widespread use of nonradioactive RNA probes for use in Northern blotting is the difficulty in stripping these probes from nylon membranes after hybridization. In this report we describe two protocols for stripping digoxigenin (Dig)-labeled RNA probes from nylon membranes. One protocol utilizes a phosphate-buffered formamide stripping solution to remove nonchemically modified (regular) RNA probes while the other method utilizes strippable probes that were produced with a chemically modified nucleotide (CTP) and removed by a specific stripping solution. This latter method was developed by Ambion Inc. and is called Strip-EZ™. We also describe a protocol for the detection of two separate rat mRNAs using both biotin and digoxigenin-labeled RNA probes that does not require stripping the membrane after hybridization. Finally, we describe the use of another new labeling technology, called ChemLink™, that quickly and conveniently labels RNA for use in Northern blotting.

Additional Material: 3 Ill.