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  • 1995-1999  (2)
  • 1915-1919
  • DNA curvature  (1)
  • Key words Green-fluorescent protein  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells (1999)
    Springer
    Plant cell reports 18 (1999), S. 707-714 
    Details
    ISSN: 1432-203X
    Keywords: Key words Green-fluorescent protein ; Transformation ; Particle bombardment ; Agrobacterium ; Sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Early detection of plant transformation events is necessary for the rapid establishment and optimization of plant transformation protocols. We have assessed modified versions of the green fluorescent protein (GFP) from Aequorea victoria as early reporters of plant transformation using a dissecting fluorescence microscope with appropriate filters. Gfp-expressing cells from four different plant species (sugarcane, maize, lettuce, and tobacco) were readily distinguished, following either Agrobacterium-mediated or particle bombardment-mediated transformation. The identification of gfp-expressing sugarcane cells allowed for the elimination of a high proportion of non-expressing explants and also enabled visual selection of dividing transgenic cells, an early step in the generation of transgenic organisms. The recovery of transgenic cell clusters was streamlined by the ability to visualize gfp-expressing tissues in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050647
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    GATC motifs may alter the conformation of DNA depending on sequence context and N6-adenine methylation status: possible implications for DNA-protein recognition (1998)
    Springer
    Molecular genetics and genomics 258 (1998), S. 488-493 
    Details
    ISSN: 1617-4623
    Keywords: Key words GATC ; dam methylation ; DNA curvature ; IHF ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract As part of our analysis of the role of a uniquely clustered set of dam methylation sites (the motif GATC) within the origin of DNA replication in Escherichia coli, we have studied the effect of GATCs in various methylation states on the intrinsic curvature of DNA. We have designed a set of DNA linkers and used commercially available linkers containing GATC motifs. The linkers were ligated and the electrophoretic mobility of the resulting multimers in different states of methylation was tested relative to reference fragments. We report that properly phased GATCs in certain sequence environments modulate DNA curvature and that these effects may be enhanced by N6-adenine methylation of the GATCs. These structural alterations may in turn affect DNA-protein interactions, especially those involving proteins that rely on both primary sequence and structure for recognition. We present an example, where introduction of a GATC within an integration host factor (IHF) binding site, which does not alter the consensus sequence, reduces the binding affinity of the protein for the modified site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050759
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    Paper (German National Licenses)
    Fulltext
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