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Expression of glycoprotein-processing enzymes in Escherichia coli as fusion proteins (1997)

Moran, D.G. ; Yoshida, S. ; Fujiyama, K. ; [et al.]

Springer

World journal of microbiology and biotechnology 13 (1997), S. 265-267

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ISSN: 1573-0972

Keywords: β-1,4-galactosyltransferase ; fusion protein ; glycoprotein processing ; maltose binding protein ; man9-mannosidase ; polymerase chain reaction

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract A truncated Man9-mannosidase gene and a full-length β-1,4-galactosyltransferase gene were isolated from human kidney and placenta cDNAs, respectively. Both genes were cloned in plasmid pMAL-c2 to produce fusions with maltose-binding protein. Fusion products were purified by affinity chromatography. Purified enzymes were assayed using pyridyl-amino labelled oligosaccharides as substrates and analysed by HPLC.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1018570604788

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