ISSN:
0006-3525
Keywords:
15N-nmr
;
13C-nmr
;
specific labeling
;
13C tags
;
nucleoside synthesis
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
We describe methods to introduce 13C specifically to the C2 or C8 positions of 15N-multilabeled purines, thereby permitting “13C tagging” of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15N-multilabeled monomers into a given DNA or RNA fragment, with and without appropriate 13C tags. This approach increases the number of 15N-nmr resonances that can be unambiguously distinguished and thus the amount of information from a single synthesis and a single nmr experiment. © 1998 John Wiley & Sons, Inc. Biopoly 48: 57-63, 1998
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
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