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Digitale Medien

Use of 13C tags with specifically 15N-labeled DNA and RNA (1998)

Abad, José-Luis ; Shallop, Anthony J. ; Gaffney, Barbara L. ; [weitere]

New York : Wiley-Blackwell

Biopolymers 48 (1998), S. 57-63

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Details

ISSN: 0006-3525

Schlagwort(e): 15N-nmr ; 13C-nmr ; specific labeling ; 13C tags ; nucleoside synthesis ; Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: We describe methods to introduce 13C specifically to the C2 or C8 positions of 15N-multilabeled purines, thereby permitting “13C tagging” of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15N-multilabeled monomers into a given DNA or RNA fragment, with and without appropriate 13C tags. This approach increases the number of 15N-nmr resonances that can be unambiguously distinguished and thus the amount of information from a single synthesis and a single nmr experiment. © 1998 John Wiley & Sons, Inc. Biopoly 48: 57-63, 1998

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

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