ISSN:
1438-2199
Keywords:
Amino acids
;
Hydroxyproline
;
Dabsyl-chloride
;
Urine
;
High-performance liquid chromatography
;
o-Phthalaldehyde
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In order to analyse hydroxyproline (HYP) in urine, a high-performance liquid chromatographic method was modified. The primary amino groups were blocked with o-phthalaldehyde, and then the secondary amino groups were derivatized with 4-dimethylaminoazobenzene-4′-sulphonyl chloride. In addition, the dabsylated samples were treated with ethyl acetate to obtain a simple elution profile in high-performance liquid chromatography. The dabsyl-HYP and -proline were eluted at 4.7 min and 8.0 min, respectively. The chromatographic analysis was completed within 10 min, including the time needed for reequilibration of the column. Using the present method, the concentration of HYP in urine was determined to 260 ± 6µmol/l.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00806559
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