ISSN:
1573-4986
Keywords:
Influenza virus
;
sialidase specificity
;
gangliosides
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The sialidase of swine influenza A viruses of N1 and N2 subtypes, isolated from 1930 to 1992, was studied for substrate specificity with ganglio-series, lacto-series type II and GM3 gangliosides containing Neu5Acα2-3Gal, Neu5Gcα2-3Gal and Neu5Acα2-6Gal linkages. All viral sialidases tested showed that the activity for hydrolysing substrates with Neu5Acα2-3Gal was higher than the activities with Neu5Gcα2-3Gal and Neu5Acα2-6Gal linkages. When GM1b, GM3 and sialylparagloboside were used as substrates, the earliest strain (A/Wisconsin/15/30 H1N1, isolated in 1930) showed the activity ratio of Neu5Acα2-6Gal to Neu5Acα2-3Gal to be 0.13:0.2, and the ratio Neu5Gcα2-3Gal/Neu5Acα2-3Gal to be 0.19:0.37, while those strains isolated from 1978 to 1992 exhibited ratios of 0.29:0.58 for Neu5Acα2-6Gal/Neu5Acα2-3Gal and 0.51:0.76 for Neu5Gcα2-3Gal/Neu5Acα2-3Gal. The above results indicate that the substrate specificities of sialidases from swine influenza A viruses towards sialyl linkages and the molecular species of sialic acid are related to the year of isolation, i.e. strains isolated after 1978 exhibited higher activity towards Neu5Acα2-6Gal and Neu5Gcα2-3Gal linkages when compared with strains isolated in an earlier year, 1930.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731360
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