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  • 1
    Electronic Resource
    Electronic Resource
    The low-molecular-weight glutenin subunit proteins of primitive wheats. II. The genes from A-genome species (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 126-134 
    Details
    ISSN: 1432-2242
    Keywords: Key words Low-molecular-weight glutenin subunit proteins ; Gene sequence ; Expression in bacteria ; A genome wheats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Three accessions of T. boeoticum were selected for the cloning and sequencing of novel low-molecular-weight glutenin subunit (LMW-GS) genes, based on the results of SDS-PAGE and PCR analyses of the LMW-GS diversity in A-genome wheat (Lee et al. 1998 a). A comparison of the nucleotide and deduced amino-acid sequences of three cloned genes, LMWG-E2, LMWG-E4 and LMWG-AQ1, both to each other and to other known LMW-GS genes was carried out. The N-terminal domains showed one variable position; GAG (coding for a glutamic acid) for the E-type, and GAT (coding for an aspartic acid) for the Q-type. The comparisons of the LMW-GSs in the literature and this paper define three different types of N-terminal sequences; METSCIPGLERPW and MDTSCIPGLERPW from the durum and A-genome wheats, and METRCIPGLERPW from the hexaploid and D-genome wheats. The repetitive domains were AC-rich at the nucleotide level and coded for a large number of glutamine residues; this region showed 16 variable positions changing 12 amino-acid residues, three triple nucleotide deletions/additions, a large deletion of 18 nucleotides in LMWG-E4 and a deletion of 12 nucleotides in LMWG-E2. In the C-terminal domains 26 variable positions were found and 12 of these mutations changed amino-acid residues; no deletions/ additions were present in this region. It was shown that the LMWG-E2 and LMWG-E4 genes could be expressed in bacteria and this allowed the respective protein products to be related back to the proteins defined as LMW-GSs in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051049
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  • 2
    Electronic Resource
    Electronic Resource
    The low-molecular-weight glutenin subunit proteins of primitive wheats. I. Variation in A-genome species (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 119-125 
    Details
    ISSN: 1432-2242
    Keywords: Key words Low-molecular-weight glutenin subunits ; A genome wheats ; Tris-Tricine PAGE ; Variation in genes by PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A Tris-Tricine gel-electrophoresis system (Schaegger and von Jagow 1987), combined with a gradient gel, has been employed to provide an improved resolution of the B and C low-molecular-weight glutenin subunits (LMW-GSs) found in the endosperm of wheat grain. The gel system was used to document the variation in the gluten subunit proteins present in A-genome diploid wheats. The majority of LMW-GSs found in the A-genome diploid wheats were not present in normal bread wheats; the data suggest that they represent a rich source of new variation for the LMW-GSs which are considered to be very important in modulating wheat flour-processing properties. The analysis of variation in the nature of the LMW-GS genes, using PCR, demonstrated that the subclass of C-subunits assayed by primers from a previously published sequence did not show as much variation as the proteins. However, the data collected suggest that sufficient variation may exist in the LMW-GS genes of A-genome diploid wheats to use them as a source of genes for altering the flour-processing properties of hexaploid wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051048
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  • 3
    Electronic Resource
    Electronic Resource
    Experimental evaluation of designs for the simulated countercurrent moving bed separator (1996)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 42 (1996), S. 683-690 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Simulated countercurrent chromatography is a continuous flow method for separation of binary mixtures or for separation of multicomponent mixtures into two fractions. Countercurrent flow is simulated by moving the feed point past several fixed adsorbent beds at a rate between the single column breakthrough time of each component. The number of columns and the location of the inlet and outlet port were varied to investigate product purity and productivity. Concentration profiles of the effluent product streams were measured. The maximum product concentration exceeds the feed concentration during part of the feed-switching cycle and drops to zero during part of the cycle. Three configurations were tested with the total number of columns varying between three and eight. For the test separation chosen, gaseous propylene and dimethyl ether on Chromosorb 101, high purity (〉 99%) product streams were obtained with an optimal four-column configuration using three desorbent streams.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690420309
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    Paper (German National Licenses)
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