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  • 1995-1999  (3)
  • Pulmonary hypertension  (2)
  • Adipose tissue  (1)
  • 1
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Chlorkohlenwasserstoffe ; Toxaphene ; Fettgewebe ; Kinder ; Langzeitstudie ; Key words Chlorinated hydrocarbons ; Toxaphenes ; Adipose tissue ; Children ; Longitudinal study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Rationale: Did concentrations of chlorinated hydrocarbons (CHC) change in the adipose tissue of children in the last 14 years? Are there yet other noxae stored in fat other than those already known? Method: Concentrations of CHC were determined quantitatively by means of gas chromatography and mass spectrometry and expressed in relation to the fat content (µg/kg fat=ppb) in specimens of adipose tissue from 1267 infants, children and adolescents from Western and Eastern Germany as well as Saratov/Russia. Results: The concentrations of hitherto known CHC have all declined substantially during recent years. However, so far unknown CHC were also detected in the adipose tissue, namely small amounts of two toxaphenes (parlar 26 and parlar 50). Conclusion: The exposure of our children to hitherto known CHC has decreased substantially in recent years. However, more sensitive methods of detection led to the identification of so far unknown potential noxae in adipose tissue, so that a complete ”all-clear” is not yet justified.
    Notes: Zusammenfassung Fragestellung: Wie haben sich die Konzentrationen der Chlorkohlenwasserstoffe (CKW) im Fettgewebe von Kindern während der letzten 14 Jahre verhalten? Sind noch andere als die bisher bekannten Schadstoffe im Fett abgelagert? Methode: In Fettgewebsproben von 1267 Säuglingen, Kindern und Adoleszenten aus West- und Ostdeutschland sowie aus Saratow, Rußland, wurden die Konzentrationen der CKW mittels Gaschromatographie bzw. Massenspektrometrie quantitativ bestimmt und auf den Fettgehalt bezogen angegeben (µg/kg Fett=ppb). Ergebnisse: Die Konzentrationen der bisher bekannten CKW sind während der letzten Jahre alle stark zurückgegangen. Es wurden aber auch bisher unbekannte CKW im Fettgewebe entdeckt, nämlich 2 Toxaphene (Parlar 26 und 50) in geringer Menge. Schlußfolgerung: Zwar ist die Belastung unserer Kinder mit den bisher bekannten CKW während der letzten Jahre deutlich zurückgegangen, doch führten empfindlichere Nachweismethoden zum Nachweis bisher unbekannter potentieller Schadstoffe im Fettgewebe, so daß eine völlige Entwarnung z.Z. noch nicht gerechtfertigt ist.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1238
    Keywords: Key words Inhaled nitric oxide ; Pulmonary hypertension ; Respiratory burst of neutrophils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract   Introduction: Inhaled nitric oxide (NO) may be beneficial in the treatment of pulmonary hypertension, both of the newborn and in the adult respiratory distress syndrome. Up to now, serious systemic side effects have not been reported. Objective: The effect of inhaled NO on superoxide anion production by neutrophils. Design: Prospective study of a consecutive series of 15 neonates and infants. Setting: Neonatal and paediatric ICUs with a total of 17 beds (university hospital). Measurements and results: Superoxide anion production was determined by a flow cytometric method using dihydrorhodamine 123 (DHR) as an oxidative probe after the priming of neutrophils with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or with Escherichia coli. The generated fluorescence was expressed as relative fluorescence intensity (RFI). Inhalation of NO for more than 24 h reduced the superoxide anion production by neutrophils stimulated with E. coli to below baseline values before NO inhalation (mRFI=158±25 vs 222±24; P=0.03). This decrease was more pronounced after more than 72 h (mRFI=133±17). At this time, superoxide anion production by fMLP-stimulated neutrophils was also decreased (mRFI=40±3, vs 57±5; P=0.03). The reduced capacity of superoxide production persisted throughout therapy with NO and lasted up to more than 4 days after the end of NO inhalation. Conclusion: The results suggest that inhalation of NO in patients with pulmonary hypertension causes reduced superoxide anion production by neutrophils stimulated with E. coli or with fMLP. To determine the clinical importance of this systemic side effect with respect to bacterial infections, a randomized controlled study is necessary.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1238
    Keywords: Inhaled nitric oxide ; Pulmonary hypertension ; Respiratory burst of neutrophils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Introduction Inhaled nitric oxide (NO) may be beneficial in the treatment of pulmonary hypertension, both of the newborn and in the adult respiratory distress syndrome. Up to now, serious systemic side effects have not been reported. Objective The effect of inhaled NO on superoxide anion production by neutrophils. Design Prospective study of a consecutive series of 15 neonates and infants. Setting Neonatal and paediatric ICUs with a total of 17 beds (university hospital). Measurements and results Superoxide anion production was determined by a flow cytometric method using dihydrorhodamine 123 (DHR) as an oxidative probe after the priming of neutrophils withN-formyl-methionyl-leucylphenylalanine (fMLP) or withEscherichia coli. The generated fluorescence was expressed as relative fluorescence intensity (RFI). Inhalation of NO for more than 24 h reduced the superoxide anion production by neutrophils stimulated withE. coli to below baseline values before NO inhalation (mRFI=158±25 vs 222±24;P=0.03). This decrease was more pronounced after more than 72 h (mRFI=133±17). At this time, superoxide anion production by fMLP-stimulated neutrophils was also decreased (mRFI=40±3, vs 57±5;P=0.03). The reduced capacity of superoxide production persisted throughout therapy with NO and lasted up to more than 4 days after the end of NO inhalation. Conclusion The results suggest that inhalation of NO in patients with pulmonary hypertension causes reduced superoxide anion production by neutrophils stimulated withE. coli or with fMLP. To determine the clinical importance of this systemic side effect with respect to bacterial infections, a randomized controlled study is necessary.
    Type of Medium: Electronic Resource
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