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  • 1
    ISSN: 1432-203X
    Keywords: Key words Somaclonal variation ; Allium cyaneum ; Fluorescence in situ hybridization ; Autotetraploid regenerants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Investigations were performed to confirm the optimal in vitro culture condition for callus induction and plant regeneration, to observe if somoclonal variation occurs among regenerated plants at the ploidy level and to analyse the chromosomal location of 5S and 18S-26S rRNA gene families using fluorescence in situ hybridization in callus-derived plants of Allium cyaneum. High-est callus initiation was achieved with bulb explants cultured on MS medium supplemented with 2,4-D and BAP at 1 mg l–1 each. A total of 195 plants was obtained when using MS medium supplemented with 1 mg l–1 NAA and 5 mg l–1 BAP; about 92% were diploid having 2n=16; 8% showed a variation in ploidy level. Using digoxigenin-labelled 5S rRNA and biotin-labelled 18S-26S rRNA gene probes, we compared the fluorescence in situ hybridization patterns of autotetraploid plants with the A. cyaneum wild type. The 5S rRNA gene sites were detected on the interstitial region in the short arm of chromosome 4 and on the interstitial region in both arms of chromosome 7. The 18S-26S rRNA gene sites were detected on the terminal region of the short arm, including the satellite of chromosome 5, as well as on a part of chromosome B. The chromosomal location of both rRNA genes in regenerated autotetraploid plants corresponded to those of the wild species.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-0350
    Keywords: Key words Brain injury ; Outcome ; Prehospital care ; Children
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract There have been relatively wide variations in the results of studies examining the outcomes of severe brain injury in children. Among the reasons for these variations in outcome, prehospital care can be considered as one of the factors. In Korea, major hospitals use an aggressive and sophisticated management policy very similar to that practiced in the West. However, effective prehospital management, such as emergency care at the scene of injury and during transport by paramedics, have not yet been established. In order to evaluate the influence of prehospital care on the outcome in severely brain-injured children, we report the outcome recorded in 73 pediatric patients treated in our hospital following severe brain injury and compare these results with those reported from centers in the western community, where well-trained paramedic care and an excellent transfer system are in operation. The overall mortality rate in our patients was 23%; 41% had a good recovery, 22% moderate disability, and 12% severe disability, and 1% remained in a vegetative state. In conclusion, we would like to suggest that prehospital care does not remarkably influence mortality rates in severely brain-injured children.
    Type of Medium: Electronic Resource
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