ISSN:
1432-2242
Keywords:
Common bean
;
Phaseolus vulgaris
;
α-Amylase inhibitor
;
Bruchid resistance
;
Genetic diversity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Variation of seed α-amylase inhibitors was investigated in 1 154 cultivated and 726 non-cultivated (wild and weedy) accessions of the common bean, Phaseolus vulgaris L. Four α-amylase inhibitor types were recognized based on the inhibtion by seed extracts of the activities of porcine pancreatic α-amylase and larval α-amylase and larval α-amylase of the Mexican bean weevil, Zabrotes subfasciatus Boheman. Of the 1 880 accessions examined most (1 734) were able to inhibit porcine pancreatic α-amylase activity, but were inactive against the Z. subfasciatus larval α-amylase; 41 inhibited only the larval α-amylase activity, 52 inhibited the activities of the two α-amylases, and 53 did not inhibit the activity of either of the α-amylases. The four different inhibitor types were designated as αAI-1, αAI2, αAI-3, and αAI-0, respectively. These four inhibitor types were identified by the banding patterns of seed glycoproteins in the range of 14–20 kDa by using SDSpolyacrylamide gel electrophoresis. Additionally, four different banding patterns were recognized in accessions with αAI-1, and were designated as αAI-1a, 1b, 1c, and 1d. Two different patterns of the accessions lacking an α-amylase inhibitory activity were identified and designated as αAI-0a and αAI-0b. The largest diversity for seed α-amylase inhibitors was observed in non-cultivated accessions collected from Mexico where all eight inhibitor types were detected. The possible relationships between the variation of seed α-amylase inhibitors and bruchid resistance are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00221985
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