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  • 1995-1999  (4)
  • Organotypic culture  (2)
  • Azolla  (1)
  • HUMCD4  (1)
Datenquelle
Materialart
Erscheinungszeitraum
  • 1995-1999  (4)
Jahr
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Forensic evaluation of HUMCD4: An Italian database (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 49-51 
    Details
    ISSN: 1437-1596
    Schlagwort(e): PCR ; HUMCD4 ; Identification ; Paternity testing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract The YTTTC pentanucleotide short tandem repeat polymorphism HumCD4 was studied in an Italian population sample. PCR products were compared to an allelic ladder by manual PAGE and silver staining. A total of 6 alleles ranging from 5 to 12 repeats were represented in the analysed sample, of which 3 alleles (10, 6 and 5 repeats) were predominant and displayed a combined frequency of 0.91. Successful amplification was obtained from different sources such as blood and urine stains, teeth and paraffin embedded tissues. Results were also determined in cases of severely degraded DNA. We consider that the HUMCD4 polymorphism may be a useful tool for individual identification, paternity testing, population studies and have also employed this locus to monitor engraftment of bone marrow transplantation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01369604
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Biomass characterization of Azolla filiculoides grown in natural ecosystems and wastewater (1999)
    Springer
    Hydrobiologia 415 (1999), S. 323-327 
    Details
    ISSN: 1573-5117
    Schlagwort(e): biofilter ; productivity ; phosphorus ; water quality ; Azolla
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The aquatic pteridophyte Azolla, a small-leaf floating plant, which lives in symbiosis with a nitrogen fixing cyanobacteria, Anabaena azollae, was widespread throughout water channels and hydrographic basins of Portugal. Azolla is also the aim of a study for its utilization as a biofilter for wastewater purification, namely for phosphorus removal (± 36%). The goal of this work is to compare the growth characteristics and biomass composition of this water fern in natural ecosystems with those obtained in some wastewaters. Plant growth rate (0.107± 0.037 d-1) and productivity (5.8 g dw m-2 d-1) suggest that Azolla can grow well in partially treated domestic wastewater, but not in diluted pig wastes. This fact, associated to its biomass composition, namely in phosphorus content (1.38 ±0.20%), increase the possibility of this plant being used to improve wastewater discharge quality. It may also be possible to use the biomass as a biofertiliser or as a feed supplement for aquatic and terrestrial animals due to its protein, crude fiber and mineral content.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003824426183
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Ultrastructural studies of the myenteric plexus and smooth muscle in organotypic cultures of the guinea-pig small intestine (1995)
    Springer
    Cell & tissue research 280 (1995), S. 627-637 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Myenteric plexus ; Smooth muscle ; Organotypic culture ; Ultrastructure ; Intestine ; small ; Guinea-pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. External muscle and myenteric plexus from the small intestine of adult guinea-pigs were maintained in vitro for 3 or 6 days. Myenteric neurons and smooth muscle cells from such organotypic cultures were examined at the electron-microscopic level. An intact basal lamina was found around the myenteric ganglia and internodal strands. Neuronal membranes, nuclei and subcellular organelles appeared to be well preserved in cultured tissues and ribosomes were abundant. Dogiel type-II neurons were distinguishable by their elongated electron-dense mitochondria, numerous lysosomes and high densities of ribosomes. Vesiculated nerve profiles contained combinations of differently shaped vesicles. Synaptic membrane specializations were found between vesiculated nerve profiles and nerve processes and cell bodies. The majority of nerve fibres were well preserved in the myenteric ganglia, in internodal strands and in bundles running between circular muscle cells. No detectable changes were found in the ultrastructure of the somata and processes of glial cells. Longitudinal and circular muscle cells from cultured tissue had clearly defined membranes with some close associations with neighbouring muscle cells. Caveolae occurred in rows that ran parallel to the long axis of the muscle cells. These results indicate that the ultrastructural features of enteric neurons and smooth muscle of the guinea-pig small intestine are well preserved in organotypic culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318365
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Ultrastructural studies of the myenteric plexus and smooth muscle in organotypic cultures of the guinea-pig small intestine (1995)
    Springer
    Cell & tissue research 280 (1995), S. 627-637 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Myenteric plexus ; Smooth muscle ; Organotypic culture ; Ultrastructure ; Intestine, small ; Guinea-pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract External muscle and myenteric plexus from the small intestine of adult guinea-pigs were maintained in vitro for 3 or 6 days. Myenteric neurons and smooth muscle cells from such organotypic cultures were examined at the electron-microscopic level. An intact basal lamina was found around the myenteric ganglia and internodal strands. Neuronal membranes, nuclei and subcellular organelles appeared to be well preserved in cultured tissues and ribosomes were abundant. Dogiel type-II neurons were distinguishable by their elongated electron-dense mitochondria, numerous lysosomes and high densities of ribosomes. Vesiculated nerve profiles contained combinations of differently shaped vesicles. Synaptic membrane specializations were found between vesiculated nerve profiles and nerve processes and cell bodies. The majority of nerve fibres were well preserved in the myenteric ganglia, in internodal strands and in bundles running between circular muscle cells. No detectable changes were found in the ultrastructure of the somata and processes of glial cells. Longitudinal and circular muscle cells from cultured tissue had clearly defined membranes with some close associations with neighbouring muscle cells. Caveolae occurred in rows that ran parallel to the long axis of the muscle cells. These results indicate that the ultrastructural features of enteric neurons and smooth muscle of the guinea-pig small intestine are well preserved in organotypic culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318365
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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