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  • 1995-1999  (3)
  • Bacillus cereus  (1)
  • Cytoplasmic determinants  (1)
  • Key words: Esophageal carcinoma—MRI sagittal image—Anteroposterior diameter—T factor.  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Studies on the active sites ofBacillus cereus sphingomyelinase substitution of some amino acids by site-directed mutagenesis (1995)
    Springer
    Amino acids 9 (1995), S. 293-298 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; Bacillus cereus ; Sphingomyelinase ; Pancreatic DNase I ; Replacement of alanine ; Replacement of histidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Chemical modifications suggested that acidic amino acids such as aspartic and glutamic acids are involved in the active sites ofBacillus cereus sphingomyelinase. Among aspartic acid residues in the conserved regions of this enzyme, Asp-126, Asp-156, Asp-233 and Asp-295 were converted to glycine by site-directed mutagenesis. According to prediction on structural similarity to pancreatic DNase I, His-151 and His-296 were also converted to alanine. The Asp and His mutants, D126G, D156G, D233G, D295G, H151A and H296A, were produced inBacillus brevis 47, a protein-hyperproducing strain. The catalytic activities of D295G, H151A and H296A were completely abolished, and sphingomyelin-hydrolyzing activity of D126G or D156G was reduced by more than 50%. The activity of D126G towardp-NPPC was comparable to that of the wild-type, while D156G catalyzed the hydrolysis of HNP andp-NPPC more efficiently than the wild-type. Hemolytic activities of the mutants were parallel to their sphingomyelin-hydrolyzing activities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00805960
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Thoracic esophageal carcinoma: evaluation in the sagittal section with magnetic resonance imaging (1997)
    Springer
    Abdominal imaging 22 (1997), S. 20 -23 
    Details
    ISSN: 1432-0509
    Keywords: Key words: Esophageal carcinoma—MRI sagittal image—Anteroposterior diameter—T factor.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Among 54 patients with thoracic esophageal carcinoma (53 squamous cell carcinoma, 1 adenosquamous carcinoma), the usefulness of MR sagittal imaging for evaluating local extent (T factor) was examined by measuring the anteroposterior (AP) diameter of the tumor. The MR examination was performed using ECG gated scans with a 3 mm slice at 1.5 Tesla. T1-weighted sagittal images were obtained. In 10 patients the appearance of the esophagus was normal, and in 44 patients a tumor was detected in the sagittal section. Thirty-seven lesions were histologically proved (Tis, 1; T1, 3; T2, 3; T3, 15; T4, 15). Lesions classified as 〈T2 were not visible, and the appearance of the esophagus was normal. The tumors detected in sagittal section were T3 (15 tumors) or T4 (15 tumors). The mean diameter of T4 lesions (34.0 ± 7.9 mm, range 24–57 mm) was larger than that of T3 lesions (25.0 ± 4.4 mm, range 18–33 mm) (p 〈 0.05). Only two masses with an AP diameter 〉 30 mm were resected. They were located in the lower esophagus. It is concluded that lesions detected in MR sagittal images might be T3 or T4 tumors, and masses of 〉30 mm might extend to adjacent organs, although two tumors with an AP diameter 〈 25 mm also invaded the trachea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002619900132
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of cytoplasmic determinants in unfertilized eggs of the ascidian Halocynthia roretzi (1996)
    Springer
    Development genes and evolution 206 (1996), S. 297-304 
    Details
    ISSN: 1432-041X
    Keywords: Key words Ascidian embryogenesis ; Polarity of unfertilized egg ; Cell fusion ; Cytoplasmic transfer ; Cytoplasmic determinants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cytoplasmic determinants that specify the fate of endoderm, muscle and epidermis cells are known to be localized in specific areas of fertilized eggs of ascidians. The presence of such cytoplasmic determinants in unfertilized eggs was demonstrated in previous studies, but no information has yet been proved about their distribution. To investigate the distribution of cytoplasmic determinants in unfertilized eggs, we devised a method for distinguishing the polarity of unfertilized eggs using vital staining and we performed cytoplasmic-transfer experiments by fusing blastomeres and cytoplasmic fragments from various identified regions of unfertilized eggs. Cytoplasmic fragments, that contained cortical and subcortical material, from five different positions along the animal-vegetal axis were prepared, and they were fused with a4.2 (presumptive-epidermis) or A4.1 (non-epidermis) blastomeres. The ectopic development of endoderm, muscle and epidermis cells that was promoted by the transplanted cytoplasm was assessed by examining the expression of alkaline phosphatase (ALP), myosin and epidermis-specific antigen, respectively. Differentiation of endoderm and muscle was observed at higher frequencies as cytoplasmic fragments closer to the vegetal pole were transplanted. Conversely, formation of epidermis was observed at higher frequencies as cytoplasmic fragments closer to the animal pole were transplanted. The results suggest that, in cortical and subcortical regions of unfertilized ascidian eggs, endoderm and muscle determinants are widely distributed along a gradient, with maximum activity at the vegetal pole, whilst epidermis determinants are also distributed along a gradient but with maximum activity at the animal pole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050056
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    Paper (German National Licenses)
    Fulltext
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