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  • 1995-1999  (3)
  • Isozyme  (2)
  • Betula pendula  (1)
  • Microinjection
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 527-529 
    ISSN: 1432-1335
    Keywords: Nuclear protein tyrosine kinase ; Hepatocarcinogensis ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The activity changes of isozymes of nuclear protein tyrosine kinase (nPTK) during chemically induced hepatocarcinogenesis in rats was studied. Two isozymes were isolated from rat liver by using ion exchange chromatography. These two isozymes were designated as nPTK-I and nPTK-II, and were found to be minor and major components of total nPTK respectively. These proved to be two different enzymes due to their different characteristics, such as molecular weights, electrical charges and kinetics. The specific activity nPTK-I and its percentages in total nPTK significantly increased in preneoplastic stage (week 10) and slightly elevated further in neoplastic stage (week 18). The specific activity of the nPTK-II moderately increased at week 10, but compared to its value at week 10, it decrease at week 18, resulted in gradual decrease of its percentage in total nPTK. These results indicate that rat hepatic nPTK-I and nTPK-II may be related to neoplastic and preneoplastic stages of rat liver respectively.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 527-529 
    ISSN: 1432-1335
    Keywords: Key words Nuclear protein tyrosine kinase ; Hepatocarcinogensis ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The activity changes of isozymes of nuclear protein tyrosine kinase (nPTK) during chemically induced hepatocarcinogenesis in rats was studied. Two isozymes were isolated from rat liver by using ion exchange chromatography. These two isozymes were designated as nPTK-I and nPTK-II, and were found to be minor and major components of total nPTK respectively. These proved to be two different enzymes due to their different characteristics, such as molecular weights, electrical charges and kinetics. The specific activity nPTK-I and its percentages in total nPTK significantly increased in preneoplastic stage (week 10) and slightly elevated further in neoplastic stage (week 18). The specific activity of the nPTK-II moderately increased at week 10, but compared to its value at week 10, it decrease at week 18, resulted in gradual decrease of its percentage in total nPTK. These results indicate that rat hepatic nPTK-I and nTPK-II may be related to neoplastic and preneoplastic stages of rat liver respectively.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    ISSN: 1573-5036
    Keywords: Betula pendula ; Nitrogen fertilisation ; CO2 concentration ; Carbon isotopic fractionation ; Lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of nitrogen (N) fertilisation and elevated [CO2] on lipid biosynthesis and carbon isotope discrimination in birch (Betula pendula Roth.) transplants were evaluated using seedlings grown with and without N fertiliser, and under two concentrations of atmospheric CO2 (ambient and ambient+250 μmol mol-1) in solar dome systems. N fertilisation decreased n-fatty acid chain length (18:0/16:0) and the ratios of α-linolenate (18:2)/linoleate (18:1), whereas elevated [CO2] showed little effect on n-fatty acid chain length, but decreased the unsaturation (18:2+18:1)/18:0. Both N fertilisation and elevated [CO2] increased the quantity of leaf wax n-alkanes, whilst reducing that of n-alkanols by 20–50%, but had no simple response in fatty acid concentrations. 13C enrichment by 1–2.5‰ under N fertilisation was observed, and can be attributed to both reduced leaf conductance and increased photosynthetic consumption of CO2. Individual n-alkyl lipids of different chain length show consistent pattern of δ13C values within each homologue, but are in general 5–8‰ more depleted in 13C than the bulk tissues. Niether nitrogen fertilisation and elevated CO2 influenced the relationship between carbon isotope discrimination of the bulk tissue and the individual lipids.
    Type of Medium: Electronic Resource
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