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  • 1995-1999  (3)
  • radioimmunoassay  (2)
  • Chihuahan Desert  (1)
  • Biliary Excretion
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 20 (1995), S. 137-146 
    ISSN: 1432-0789
    Keywords: Larrea tridentata ; Simulated rainfall ; Precipitation exclusion ; Soil drying effects ; Analysis of covariance ; Chihuahan Desert ; Mineralization rate ; Field capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Irrigation and rain-out shelters were used to simulate precipitation patterns of wet and dry years in the northern Chihuahuan Desert. Irrigation provided approximately double the long-term average monthly precipitation. Rain was excluded during the wet season, July-October, to simulate a dry year. N net mineralization in laboratory incubations was undectable at calculated water potentials less than -1 MPa. Witb increasing moisture, mineralization gradually rose to the highest observed rates near field capacity. There was no mineralization maximum at moisture contents below field capacity. Irrigation significantly increased the water potential and rainfall exclusion reduced water potentials to less than-8 MPa. The general absence of important irrigation effects may have resulted from the high natural precipitation during the experiment or because irrigation inputs were insufficient to increase microbial activity during very dry periods. Precipitation exclusion reduced ion capture during the warm-wet season. After allowing precipitation inputs to resume, NH 4 + -N capture was increased in the cool-dry seasons of both 1987–1988 and 1988–1989. NH 4 + -N capture more than doubled that predicted from the overall covariance of moisture input and ion capture, suggesting increased availability of N. An unusually hot, dry period in May and June 1989 was followed by a threeto fourfold increase in the warm-wet season NO 3 − +NO2−N capture compared to 1988. These data suggest that short droughts of about 3 months in length (both simulated and natural) increased N availability relative to moisture availability.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 30 (1995), S. 165-176 
    ISSN: 0739-4462
    Keywords: Lepidoptera ; radioimmunoassay ; enzyme immunoassay ; equilibrium dialysis ; monoclonal antibody ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Numerous studies have demonstrated regulation of specific lepidopteran proteins by pharmacological doses of insect juvenile hormone (JH). In this study, topical application of a 1 pg dose of JH I to fourth stadium larvae of the black (bl) mutant strain of the tobacco hornworm, Manduca sexta, induced a 50% increase in the titer of hemolymph juvenile hormone binding protein (hJHBP). Radioimmunoassay confirmed that JH titers were lower in bl larvae than in wild-type larvae at the time of JH treatment. Enzyme immunoassay analysis of hJHBP titers demonstrated that regulation by JH I was dose-dependent at doses up to 10 pg and that the response was saturated above 100 pg. Western blotting and equilibrium dialysis confirmed these results and demonstrated that hJHBP from bl larvae had the same molecular mass and displayed the same affinity for JH I as hJHBP isolated from wild-type larvae. Time course studies showed that regulation was complex: 1 2 h after JH I treatment, hJHBP titers were twofold lower in treated than in control bl larvae, while 44 h after treatment they were twofold higher. JH I regulation of hJHBP titers in bl larvae was independent of changes in total hemolymph protein. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 30 (1995), S. 295-306 
    ISSN: 0739-4462
    Keywords: radioimmunoassay ; JH I ; JH II ; JH III ; hemolymph ; insect hormone ; Manducasexta sexta ; Hyalophora cecropia ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Recent refinements in juvenile hormone radioimmunoassay technology now make this method significantly more sensitive and easier to use. Rabbit poly-clonal antisera against (10R) JH III and racemic JH II have been developed to determine hemolymph hormone titers in the low picogram range. The antisera display minimal cross-reactivity with JH metabolites, JH analogs, and hemolymph lipids. One antiserum recognizes racemic JH I, II, and (10R) III almost equivalently, exhibiting 50% displacement between 100 and 130 pg per tube. Another antiserum is JH II-specific and exhibits 50% displacement at 35 pg per tube. Assay sensitivity has been enhanced by using (10R,11S) [methyl-3H]-JH II of very high specific activity (〉 80 Ci/mmol) generated with Hyalophora cecropia accessory gland S-adenosylmethionine transferase and S-[methyl-3H]-adenosyl-L-methionine. Preparation of biological samples has been simplified with overall recoveries of JH from hemolymph ranging between 60 and 75%. © 1995 Wiley-Liss, Inc.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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