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  • 1995-1999  (6)
  • callus  (3)
  • dissolved organic carbon (DOC)  (2)
  • Biomass increase  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 263-268 
    ISSN: 1573-5044
    Keywords: Auxin ; callus ; cytokinin ; fennel ; histological analysis ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus induction and morphogenic response of several fennel populations were determined by genotype and hormonal treatment. 100% callus formation occurred only in the Francia Pernod population under the action of 2, 4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid and kinetin. Only this last hormonal treatment induced shoot regeneration. Plant regeneration was observed especially in Francia Pernod population. Ths calluses grown in presence of 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid plus kinetin, showed considerable differences at cytological and histological level which were correlated to their different morphogenic capability.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 50 (1997), S. 125-129 
    ISSN: 1573-5044
    Keywords: callus ; metal tolerance ; shoot regeneration ; Solanaceae ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Manganese toxicity to germination, callus induction and shoot regeneration was studied on three cultivars of Nicotiana tabacum: BEL W3, Burley 21, Bright NC 944. All materials were cultured on MS solid medium containing 0.1 (control), 2, 5, 10, 15 and 20 mM Mn, to which 5 µM NAA and 5 µM kinetin were added for callus induction and shoot regeneration. Mn toxicity to callus growth was tested using habituated callus of Nicotiana bigelovii var. bigelovii grown on MS medium without growth regulators. Mn concentrations higher than 2 mM were toxic for germination, and concentrations higher than 5 mM were toxic for callus induction, shoot regeneration and callus growth. Among the cultivars examined, Bright tobacco appeared more tolerant to high Mn concentrations during callus formation and shoot regeneration. However, many regenerated plants capable of growing in vitro in the presence of 2 and 5 mM Mn were obtained.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-515X
    Keywords: climate change ; dissolved organic carbon (DOC) ; forested catchments ; nutrient export ; Precambrian Shield streams ; storm runoff
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Dissolved organic carbon (DOC) concentrations and DOC export arestudied during storms to examine the relationship between DOCconcentration and stream discharge and to assess the importance of stormson DOC export. Storms were monitored in seven subcatchments within twosmall watersheds (Harp 4--21 and Harp 3A) on the Precambrian Shield inCentral Ontario, Canada. Stream DOC concentrations increase during stormsby as much as 100% and 410% in Harp3A and Harp 4--21 respectively. The seasonal regression between DOC andstream discharge is significant in subcatchments without wetlands(r2 〉 0.7) but is not significant in thetwo subcatchments with small wetland areas (r2 〈0.06). On average, regressions based on weekly data yield accurate estimatesof DOC export but the variation in regressions among individual storms andthe small number of high DOC samples result in uncertainties of more than30% in DOC export. The period-weighted calculation ofDOC export from weekly data underestimates export by 14%and 22% in Harp 3A and Harp 4--21 respectively. Stormswere responsible for 57% to 68% of theDOC export in the autumn and 29% to 40%of the DOC export in the spring. A single large storm accounted for31% of the autumn DOC export in Harp 3A. The importanceof individual storms for DOC export and the variation in the relationshipbetween DOC and stream discharge among storms make it difficult to predictthe effects of climate change on DOC export and DOC concentrations.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-515X
    Keywords: carbon cycling ; dissolved organic carbon (DOC) ; forested catchments ; nutrient export ; Precambrian Shield streams ; storm runoff
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Dissolved organic carbon (DOC) concentrations and export were studied in two small catchments in central Ontario to examine DOC sources and to assess the hypothesis that organic matter adjacent to the stream is a significant contributor of DOC during storms. Different DOC dynamics and exports were observed according to the depth of the riparian water table. In Harp 4-21, riparian flowpaths were predominantly through A and upper B soil horizons and riparian soils contributed between 73 and 84% of the stream DOC export during an autumn storm. In Harp 3A, riparian flowpaths were predominantly through lower B horizons. Consequently, riparian soils were less important and hillslopes contributed more than 50% of the stream DOC export in subcatchments without wetlands during storms. Wetlands and adjacent soils contributed significantly to DOC export in Harp 3A; 8% of the total catchment area exported 32 to 46% of the storm runoff DOC. DOC export dynamics in wetlands and riparian soils were distinctly different. In wetlands, transport was affected by leaching and flushing of DOC at the wetland surface leading to lower DOC concentrations with successive storms. In riparian soils, groundwater flowpaths were more important and stronger positive relationships between discharge and DOC concentration were observed. Precipitation, throughfall and stemflow were minor sources of stream DOC during storms and contributed less than 20% of the total export.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5044
    Keywords: callus ; copper toxicity ; N. tabacum ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Nicotiana tabacum L. var. BEL W3 copper (Cu) at concentrations higher than 50 μM significantly inhibited callus growth and shoot regeneration. After 5–6 months of culture only a few morphogenic callus lines survived in the presence of 100 μM Cu. These calluses showed the capacity to grow and regenerate shoots through successive subcultures on medium containing 100 μM Cu. The 100 μM Cu-tolerant shoots, in comparison to regenerated control shoots, formed roots only when cultured in the presence of 100 μM Cu. From five independent Cu-tolerant callus lines in a culture period of 4–5 months more than 50 plants (defined ‘tolerant’) able to grow in presence of 100 μM Cu were obtained. These plants showed normal xylem tissue formation while control regenerated plants growing in normal Cu MS content (0.1 μM) had few xylem elements in the central cylinder. No difference as far chlorophyll content and chloroplast structure was found among Cu-tolerant and control plants. In Cu-tolerant plants, dry matter production was higher than in controls, particularly in roots.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 67-72 
    ISSN: 1573-5044
    Keywords: Biomass increase ; chromosome count ; growth regulators ; regeneration ; tissue culture ; wormwood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures were initiated from micropropagated Artemisia absinthium plantlets on MS basal medium supplemented with different concentrations of BA, Kn, NAA, IAA and 2,4-d in combination or singly. Supplementing the medium with low doses of both BA in combination with NAA, and Kn in combination with NAA enhanced the growth rate of callus cultures. However, cultures grew slowly following the second subculture and the majority turned brown and died within the next month. Initiation of root and shoot primordia occured directly from leaf explants cultured on 1.81 μM 2,4-d, while adventitious shoot formation from callus was observed occasionally when BA was added to the medium in combination with IAA. Furthermore, medium containing 2.22 μM BA and 2.69 μM NAA stimulated both callus growth and organogenesis on some callus cultures derived from leaves and stems of young stock material. The best results were obtained with leaf explants. Cytological analysis of root meristems revealed that all regenerants were diploid (2n=18), as expected.
    Type of Medium: Electronic Resource
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